Inner Mongolia ZhongXing Agriculture and Animal Husbandry Development Co., Ltd., Ulanqab, China.
Inner Mongolia Mang Lai Food Co., Ltd., Hohhot, China.
Sci Rep. 2019 Oct 25;9(1):15376. doi: 10.1038/s41598-019-51831-4.
Morchella are macrofungi and are also called morels, as they exhibit a morel-like upper cap structure. Morels contain abundant essential amino acids, vitamins and biologically active compounds, which provide substantial health benefits. Approximately 80 species of Morchella have been reported, and even more species have been isolated. However, the lack of wild Morchella resources and the difficulties associated with culturing Morchella have caused a shortage in the morels available for daily consumption. Additionally, in-depth genomic and morphological studies are still needed. In this study, to provide genomic data for further investigations of culturing techniques and the biological functions of Morchella sextelata (M. sextelata), de novo genome sequencing was carried out on the Illumina HiSeq. 4000 platform using both the Illumina 150 and PacBio systems. The final estimated genome size of M. sextelata was 52.93 Mb, containing 59 contigs and a GC content of 47.37%. A total of 9,550 protein-coding genes were annotated. In addition, the repeat sequences, gene components and gene functions were analyzed using various databases. Furthermore, the secondary metabolite gene clusters and the predicted structures of their products were analyzed. Finally, a genomic comparison of different species of Morchella was performed.
羊肚菌是大型真菌，也被称为羊肚蘑，因为它们具有羊肚菌状的上部帽状结构。羊肚菌含有丰富的必需氨基酸、维生素和生物活性化合物，具有显著的健康益处。据报道，大约有 80 种羊肚菌，还有更多的物种被分离出来。然而，由于野生羊肚菌资源的缺乏和羊肚菌培养的困难，导致日常消费的羊肚菌供应短缺。此外，还需要进行深入的基因组和形态学研究。在这项研究中，为了提供基因组数据，以进一步研究羊肚菌的培养技术和生物学功能，我们使用 Illumina 150 和 PacBio 系统在 Illumina HiSeq 4000 平台上对羊肚菌 sextelata（M. sextelata）进行了从头测序。M. sextelata 的最终估计基因组大小为 52.93Mb，包含 59 个 contigs 和 47.37%的 GC 含量。总共注释了 9550 个蛋白质编码基因。此外，还使用各种数据库分析了重复序列、基因成分和基因功能。此外，还分析了次生代谢物基因簇及其产物的预测结构。最后，对不同种羊肚菌进行了基因组比较。
