Department of Prosthetic Dentistry, Faculty of Dentistry, Nantes, France.
Department of Preclinical Biomedical Sciences, Mammalian Cell Culture Laboratory, GIGA-R, Faculty of Medicine, Liège, Belgium.
J Biomed Mater Res A. 2020 Mar;108(3):412-425. doi: 10.1002/jbm.a.36823. Epub 2019 Nov 21.
Stereolithography (SLA) is an interesting manufacturing technology to overcome limitations of commercially available particulated biomaterials dedicated to intra-oral bone regeneration applications. The purpose of this study was to evaluate the in vitro and in vivo biocompatibility and osteoinductive properties of two calcium-phosphate (CaP)-based scaffolds manufactured by SLA three-dimensional (3D) printing. Pellets and macro-porous scaffolds were manufactured in pure hydroxyapatite (HA) and in biphasic CaP (HA:60-TCP:40). Physico-chemical characterization was performed using micro X-ray fluorescence, scanning electron microscopy (SEM), optical interferometry, and microtomography (μCT) analyses. Osteoblast-like MG-63 cells were used to evaluate the biocompatibility of the pellets in vitro with MTS assay and the cell morphology and growth characterized by SEM and DAPI-actin staining showed similar early behavior. For in vivo biocompatibility, newly formed bone and biodegradability of the experimental scaffolds were evaluated in a subperiosteal cranial rat model using μCT and descriptive histology. The histological analysis has not indicated evidences of inflammation but highlighted close contacts between newly formed bone and the experimental biomaterials revealing an excellent scaffold osseointegration. This study emphasizes the relevance of SLA 3D printing of CaP-based biomaterials for intra-oral bone regeneration even if manufacturing accuracy has to be improved and further experiments using biomimetic scaffolds should be conducted.
立体光固化（SLA）是一种有趣的制造技术，可以克服专门用于口腔内骨再生应用的商业可用颗粒状生物材料的局限性。本研究的目的是评估通过 SLA 三维（3D）打印制造的两种基于钙磷（CaP）的支架的体外和体内生物相容性和成骨特性。纯羟基磷灰石（HA）和双相 CaP（HA：60-TCP：40）制造了颗粒和大孔支架。使用微 X 射线荧光、扫描电子显微镜（SEM）、光学干涉和微断层扫描（μCT）分析进行物理化学特性表征。成骨样 MG-63 细胞用于体外通过 MTS 测定评估颗粒的生物相容性，并通过 SEM 和 DAPI-肌动蛋白染色来表征细胞形态和生长，结果表明早期行为相似。为了评估体内生物相容性，使用 μCT 和描述性组织学在骨膜下颅骨大鼠模型中评估了实验支架的新骨形成和生物降解性。组织学分析没有表明炎症的证据，但强调了新形成的骨与实验生物材料之间的紧密接触，揭示了出色的支架骨整合。这项研究强调了基于 CaP 的生物材料的 SLA 3D 打印对于口腔内骨再生的相关性，即使必须提高制造精度，并且应该进行使用仿生支架的进一步实验。
