Gatti R A, Cousineau M, Naeim F, Leibold W
Tissue Antigens. 1979 Sep;14(3):213-22. doi: 10.1111/j.1399-0039.1979.tb00843.x.
We have observed that certain antisera to HLA antigens suppress the in vitro proliferation of lymphoblastoid cell lines. An antiserum to HLA-B8 demonstrated suppressor activity associated with the presence of B8 antigen on the target LCLs; this activity was removed by absorption with platelets or with B8-positive LCLs. An antiserum to HLA-DRw7 suppressed proliferation of all six DRw7-positive LCLs and none of 12 DRw7-negative LCLs; this activity was removed by absorptions with three DRw7-positive LCLs, each representing a different HLA-D allele (i.e. Dw7, 10 and 11); it could not be removed by triple serial absorptions with platelets from multiple-donor pools. These experiments indicate that the suppressor activity of this serum is specifically associated with antibodies to DRw7. Our model of LCL growth suppression by HLA antisera is easily manipulated and provides a definitive tool for further characterizing HLA antigens and antisera in a complement-independent system.
我们观察到，某些针对HLA抗原的抗血清可抑制淋巴母细胞系的体外增殖。一种针对HLA - B8的抗血清表现出与靶LCL上存在B8抗原相关的抑制活性；这种活性可通过用血小板或B8阳性LCL吸收而去除。一种针对HLA - DRw7的抗血清抑制了所有六种DRw7阳性LCL的增殖，而对12种DRw7阴性LCL均无抑制作用；这种活性可通过用三种DRw7阳性LCL吸收而去除，每种LCL代表不同的HLA - D等位基因（即Dw7、10和11）；用来自多个供体库的血小板进行三次连续吸收不能去除这种活性。这些实验表明，该血清的抑制活性与针对DRw7的抗体特异性相关。我们的HLA抗血清抑制LCL生长的模型易于操作，并为在非补体依赖系统中进一步鉴定HLA抗原和抗血清提供了一个决定性工具。
