Feighery C F, Stastny P
Immunogenetics. 1980;10(1):31-8. doi: 10.1007/BF01561550.
Cytotoxic effector cells against HLA-D-region produts were generated in cultures with HLA-A- and B-matched, HLA-D-mismatched stimulating cells. Monocytes from unrelated donors sharing HLA-D/DR antigens with the primary stimulator were used as target cells. Lysis of target cells was inhibited by addition of unlabelled monocytes having the same HLA-D/DR antigens. Inhibition of cytotoxicity was also observed with unlabelled B cells, but T lymphocytes had little effect. The distribution of the target antigens, therefore, fits the known distribution of the products of HLA-D. In other experiments, a human alloantiserum specific for HLA-DRw3 was found to inhibit cellular cytotoxicity specific for HLA-D/DRw3. Lysis by HLA-D/DRw3-specific effector cells was not inhibited by sera against HLA-DRw2 or DRw7 or by antibodies against HLA-B8 using HLA-B8 positive, DRw3 positive targer cells. A xenogeneic serum against human Ia antigens, produced in rabbits, blocked cytotoxicity directed at DRw2, DRw3 and DRw4. These results suggest that cell-mediated cyctotoxicity was directed against HLA-D/DR or very closely associated determinants.
针对 HLA - D 区产物的细胞毒性效应细胞是在与 HLA - A 和 B 匹配、HLA - D 不匹配的刺激细胞共培养时产生的。与主要刺激细胞共享 HLA - D/DR 抗原的无关供体的单核细胞用作靶细胞。加入具有相同 HLA - D/DR 抗原的未标记单核细胞可抑制靶细胞的裂解。用未标记的 B 细胞也观察到细胞毒性的抑制，但 T 淋巴细胞几乎没有作用。因此，靶抗原的分布符合 HLA - D 产物的已知分布。在其他实验中，发现一种针对 HLA - DRw3 的人同种异体抗血清可抑制针对 HLA - D/DRw3 的细胞毒性。使用 HLA - B8 阳性、DRw3 阳性靶细胞时，针对 HLA - D/DRw3 的效应细胞的裂解不受针对 HLA - DRw2 或 DRw7 的血清或针对 HLA - B8 的抗体的抑制。在兔中产生的针对人 Ia 抗原的异种血清可阻断针对 DRw2、DRw3 和 DRw4 的细胞毒性。这些结果表明细胞介导的细胞毒性是针对 HLA - D/DR 或非常紧密相关的决定簇。
