School of Biological Science and Technology, University of Jinan, Jinan 250022, PR China.
College of Marine Science, Shandong University, Weihai 264209, PR China.
Int J Syst Evol Microbiol. 2020 Jan;70(1):618-623. doi: 10.1099/ijsem.0.003800.
A golden-pigmented, Gram-strain-negative, aerobic, rod-shaped, non-flagellated and non-gliding bacterium, designated strain lm2, was isolated from activated sludge obtained from a wastewater treatment plant in Binzhou (Shandong province, PR China). Growth occurred at 15-45°C (optimum, 30 °C), in the presence of 0-5.0 % (w/v) NaCl (optimum, 0-2.0 %) and at pH 6.5-8.0 (optimum, pH 7.0-7.5). The chemotaxonomic, phenotypic and genomic traits were investigated. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain lm2 belonged to the genus , with highest sequence similarity to CC-CZW010 (97.1 %). Genome sequencing revealed a genome size of 3 611 894 bp and a G+C content of 34.9 mol%. The average nucleotide identity value and the digital DNA-DNA hybridization (dDDH) value between strain lm2 and JCM 30470 were 87.8 and 34.7 %, respectively. The major respiratory quinone was Menaquinone-6 (MK-6). The major fatty acids were iso-C, iso-C 3-OH and iso-C 9 and its polar lipids consisted of phosphatidylethanolamine (PE), unidentified lipids (L1-5) and unidentified aminolipids (AL1-4). On the basis of these data, strain lm2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is lm2 (=KCTC 72529=CCTCC AB2019126).
一株金黄色、革兰氏阴性、需氧、杆状、无鞭毛和不滑行的细菌，被命名为 lm2 菌株，从中国山东省滨州市一家污水处理厂的活性污泥中分离得到。该菌在 15-45°C（最佳温度为 30°C）、0-5.0%（w/v）NaCl（最佳浓度为 0-2.0%）和 pH6.5-8.0（最佳 pH 值为 7.0-7.5）的条件下生长。对其化学生物型、表型和基因组特征进行了研究。基于 16S rRNA 基因序列的系统发育分析表明，lm2 菌株属于 属，与 CC-CZW010 （97.1%）的相似性最高。基因组测序显示其基因组大小为 3611894bp，G+C 含量为 34.9mol%。lm2 菌株与 JCM 30470 之间的平均核苷酸同一性值和数字 DNA-DNA 杂交（dDDH）值分别为 87.8%和 34.7%。主要呼吸醌为menaquinone-6（MK-6）。主要脂肪酸为 iso-C、iso-C 3-OH 和 iso-C 9，极性脂类由磷脂酰乙醇胺（PE）、未鉴定脂类（L1-5）和未鉴定的氨基脂类（AL1-4）组成。基于这些数据，lm2 菌株被认为代表了 属的一个新种，建议将其命名为 sp. nov.。其模式菌株为 lm2（=KCTC 72529=CCTCC AB2019126）。
