Heo Jun, Won Miyoung, Lee Daseul, Kim Jeong-Seon, Han Byeong-Hak, Kim Soo-Jin, Hong Seung-Beom, Kwon Soon-Wo
Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea.
Int J Syst Evol Microbiol. 2022 Oct;72(10). doi: 10.1099/ijsem.0.005532.
Two Gram-stain-negative, aerobic, mesophilic, rod-shaped bacteria, ADR-1 and SC2-2, were isolated from Andong sikhye and dust in a pigpen, respectively. The phylogenetic tree on the basis of 16S rRNA gene sequences showed that strains ADR-1 and SC2-2 were members of the genus and revealed the highest sequence similarities to LM2 (97.6 %) and Chj707 (94.9 %), respectively. Phylogenomic treeing using 92 core genes clearly indicated that strain ADR-1 clustered with CC-CZW010, LM2 and CGMCC 1.10941, and strain SC2-2 formed a compact cluster with CCUG 49689. The digital DNA-DNA hybridization (dDDH) and orthologous average nucleotide identity (ANI) values of strain ADR-1 with the closely related species of the genus were ≤24.4 % and ≤80.7 %, and those of strain SC2-2 were ≤24.0 % and ≤77.8 %, respectively, which are well below the cut-off values of species discrimination (>70 % dDDH and >95-96 % ANI). The only respiratory quinone in both strains was menaquinone 6. The polar lipid profile of strain ADR-1 comprised phosphatidylethanolamine, four unidentified aminolipids and three unidentified lipids, while strain SC2-2 contained phosphatidylethanolamine, two unidentified aminolipids, one unidentified aminophospholipid and five unidentified polar lipids. The major fatty acids (>10 %) of strain ADR-1 were iso-C, summed feature 9 (iso-C 9c and/or C 10-methyl), iso-C 3-OH and summed feature 3 (C 7 and/or C 6), and those of strain SC2-2 were iso-C and anteiso-C. On the basis of the results of phenotypic and phylogenetic analyses, strains ADR-1 and SC2-2 represent two distinct novel species in the genus , for which the names sp. nov. (type strain ADR-1=KACC 19311=NBRC 113104) and sp. nov. (type strain SC2-2=KACC 19313=NBRC 113106) are proposed.
从安东浊酒和猪圈灰尘中分别分离出两株革兰氏阴性、需氧、嗜温、杆状细菌，ADR-1和SC2-2。基于16S rRNA基因序列构建的系统发育树表明，菌株ADR-1和SC2-2分别属于该属成员，且与LM2（97.6%）和Chj707（94.9%）的序列相似性最高。使用92个核心基因构建的系统基因组树清楚地表明，菌株ADR-1与CC-CZW010、LM2和CGMCC 1.10941聚类在一起，菌株SC2-2与CCUG 49689形成一个紧密的聚类。菌株ADR-1与该属密切相关物种的数字DNA-DNA杂交（dDDH）值和直系同源平均核苷酸同一性（ANI）值分别≤24.4%和≤80.7%，菌株SC2-2的分别≤24.0%和≤77.8%，均远低于物种区分的临界值（>70% dDDH和>95-96% ANI）。两株菌唯一的呼吸醌均为甲基萘醌6。菌株ADR-1的极性脂质谱由磷脂酰乙醇胺、四种未鉴定的氨基脂质和三种未鉴定的脂质组成，而菌株SC2-2含有磷脂酰乙醇胺、两种未鉴定的氨基脂质、一种未鉴定的氨基磷脂和五种未鉴定的极性脂质。菌株ADR-1的主要脂肪酸（>10%）为异-C、总和特征9（异-C 9c和/或C 10-甲基）、异-C 3-OH和总和特征3（C 7和/或C 6），菌株SC2-2的主要脂肪酸为异-C和anteiso-C。基于表型和系统发育分析结果，菌株ADR-1和SC2-2代表该属中两个不同的新物种，为此提议将其命名为新种（模式菌株ADR-1=KACC 19311=NBRC 113104）和新种（模式菌株SC2-2=KACC 19313=NBRC 113106）。
