sp. nov., an alkalitolerent bacterium isolated from Umiam lake, Shillong, India.

A Gram-stain-negative, motile, alkali-tolerant, swollen-rod shaped, reddish brown coloured, phototrophic bacterium designated as strain JA980, was isolated from freshwater sampled at Umiam lake, Shillong, India. Strain JA980 grew well up to pH 9.0. Respiratory quinones were ubiquinone 10 and rhodoquinone 10. The major fatty acid was C ω7/Cω6c with minor amounts of C, C, C 3-OH and C 3-OH. Strain JA980 contained bacteriochlorophyll- and carotenoids of the spirilloxanthin series. The polar lipids of strain JA980 comprised phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol, an unidentified phospholipid, unidentified amino lipids (AL1,3,4,5) and an unidentified lipid (L1). Strain JA980 had the highest (99.57 %) 16S rRNA gene sequence similarity to the type strains of ATCC17100 and JA643. The genome of strain JA980 was 3.88 Mbp with a DNA G+C content of 62.4 mol%. Based on the results of phylogenetic analyses, low DNA-DNA hybridization values (33 %), low (87 %) average nucleotide identity results, chemotaxonomic characteristics and differential physiological properties, strain JA980 could not be classified into either of the two recognized species of the genus , suggesting that it represents a novel species, for which the name sp. nov. is proposed. The type strain is JA980 (=KCTC 15697= MCC 3714= NBRC 113803).