Rahul K, Azmatunnisa M, Sasikala Ch, Ramana Ch V
Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, JNT University Hyderabad, Kukatpally, Hyderabad, 500 085, India.
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad, 500 046, India.
Int J Syst Evol Microbiol. 2015 Aug;65(8):2403-2409. doi: 10.1099/ijs.0.000277. Epub 2015 Apr 21.
A Gram-stain-negative, diesel-oil-degrading, rod-shaped bacterium (designated JC234T) was isolated from a water sample collected from diesel-oil-contaminated backwaters in Kerala, India. Strain JC234T was oxidase- and catalase-positive, and grew at 20-35 °C and at pH 7-9. Cells contained bacteriochlorophyll-a, hydroxydemethylspheroidene and three unidentified carotenoids. Growth occurred under aerobic, microaerobic and phototrophic anaerobic conditions. Strain JC234T could utilize diesel-oil as a sole source of carbon and energy. Based on the 16S rRNA gene sequence analysis, strain JC234T belonged to the genus Hoeflea within the family Phyllobacteriaceae, and was closely related to Hoeflea alexandrii AM1V30T (98.1% 16S rRNA gene sequence similarity), Hoeflea halophila JG120-1T (97.6%) and other members of the genus Hoeflea ( < 96.4 ). Strain JC234T showed 22 ± 2% and 28 ± 1.5 % DNA-DNA hybridization with Hoeflea alexandrii KCTC 22096T and Hoeflea halophila KCTC 23107T, respectively. The DNA G+C content of strain JC234T was 54.3 mol %. The major cellular fatty acids were C18 : 1ω7c/C18 : 1ω6c, C16 : 0 and C16 : 1ω7c/C16 : 1ω6c. Phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and phosphatidylglycerol were the major polar lipids. Strain JC234T contained Q10 as the predominant ubiquinone. On the basis of morphological, physiological, genetic, phylogenetic and chemotaxonomical analyses, we conclude that strain JC234T represents a novel species of the genus Hoeflea, for which the name Hoefleaolei sp. nov. is proposed. The type strain is JC234T ( = KCTC 42071T = LMG 28200T). An emended description of the genus Hoeflea is also provided.
从印度喀拉拉邦受柴油污染的死水采集的水样中分离出一株革兰氏阴性、能降解柴油的杆状细菌（命名为JC234T）。菌株JC234T氧化酶和过氧化氢酶呈阳性，在20-35 °C和pH 7-9条件下生长。细胞含有细菌叶绿素-a、羟基去甲基球形烯和三种未鉴定的类胡萝卜素。在好氧、微好氧和光合厌氧条件下均可生长。菌株JC234T可以利用柴油作为唯一的碳源和能源。基于16S rRNA基因序列分析，菌株JC234T属于根瘤菌科中的霍夫勒菌属，与亚历山大霍夫勒菌AM1V30T（16S rRNA基因序列相似性为98.1%）、嗜盐霍夫勒菌JG120-1T（97.6%）以及霍夫勒菌属的其他成员（<96.4%）密切相关。菌株JC234T与亚历山大霍夫勒菌KCTC 22096T和嗜盐霍夫勒菌KCTC 23107T的DNA-DNA杂交率分别为22 ± 2%和28 ± 1.5 %。菌株JC234T的DNA G+C含量为54.3 mol %。主要细胞脂肪酸为C18 : 1ω7c/C18 : 1ω6c、C16 : 0和C16 : 1ω7c/C16 : 1ω6c。磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰单甲基乙醇胺和磷脂酰甘油是主要的极性脂质。菌株JC234T以Q10作为主要泛醌。基于形态学、生理学、遗传学、系统发育学和化学分类学分析，我们得出结论，菌株JC234T代表霍夫勒菌属的一个新物种，为此提出名为霍夫勒油菌（Hoefleaolei sp. nov.）的名称。模式菌株为JC234T（=KCTC 42071T = LMG 28200T）。还提供了霍夫勒菌属的修订描述。
