The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, Guangdong, China.
The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, Guangdong, China.
PLoS One. 2019 Oct 29;14(10):e0223674. doi: 10.1371/journal.pone.0223674. eCollection 2019.
Aluminum (Al3+) toxicity is a typical abiotic stress that severely limits crop production in acidic soils. In this study, an RIL (recombinant inbred line, F12) population derived from the cross of Zhonghuang 24 (ZH 24) and Huaxia 3 (HX 3) (160 lines) was tested using hydroponic cultivation. Relative root elongation (RRE) and apical Al3+ content (AAC) were evaluated for each line, and a significant negative correlation was detected between the two indicators. Based on a high-density genetic linkage map, the phenotypic data were used to identify quantitative trait loci (QTLs) associated with these traits. With composite interval mapping (CIM) of the linkage map, five QTLs that explained 39.65% of RRE and AAC variation were detected on chromosomes (Chrs) Gm04, Gm16, Gm17 and Gm19. Two new QTLs, qRRE_04 and qAAC_04, were located on the same region of bin93-bin94 on Chr Gm04, which explained 7.09% and 8.98% phenotypic variation, respectively. Furthermore, the results of the expression analysis of candidate genes in the five genetic regions of the QTLs showed that six genes (Glyma.04g218700, Glyma.04g212800, Glyma.04g213300, Glyma.04g217400, Glyma.04g216100 and Glyma.04g220600) exhibited significant differential expression between the Al3+ treatment and the control of two parents. The results of qRT-PCR analysis indicated that Glyma.04g218700 was upregulated by Al3+ treatment with the hundreds-fold increased expression level and may be a candidate gene with potential roles in the response to aluminum stress. Therefore, our efforts will enable future functional analysis of candidate genes and will contribute to the strategies for improvement of aluminum tolerance in soybean.
铝（Al3+）毒性是一种典型的非生物胁迫，严重限制了酸性土壤中作物的产量。在这项研究中，使用水培培养方法对来自中黄 24（ZH 24）和华夏 3（HX 3）杂交的重组自交系（RIL）群体（160 条）进行了测试。对每条线的相对根伸长（RRE）和根尖 Al3+含量（AAC）进行了评估，并检测到这两个指标之间存在显著的负相关。基于高密度遗传连锁图谱，使用表型数据鉴定与这些性状相关的数量性状位点（QTL）。通过连锁图谱的复合区间作图（CIM），在染色体（Chrs）Gm04、Gm16、Gm17 和 Gm19 上检测到解释 RRE 和 AAC 变异的 5 个 QTL，分别解释了 39.65%和 39.65%。两个新的 QTL，qRRE_04 和 qAAC_04，位于 Chr Gm04 的 bin93-bin94 同一区域，分别解释了 7.09%和 8.98%的表型变异。此外，对 QTL 五个遗传区域候选基因表达分析的结果表明，六个基因（Glyma.04g218700、Glyma.04g212800、Glyma.04g213300、Glyma.04g217400、Glyma.04g216100 和 Glyma.04g220600）在双亲的 Al3+处理与对照之间表现出显著的差异表达。qRT-PCR 分析结果表明，Glyma.04g218700 被 Al3+处理上调，表达水平增加数百倍，可能是一个与铝胁迫反应有关的候选基因。因此，我们的努力将使未来对候选基因的功能分析成为可能，并有助于提高大豆对铝的耐受性的策略。
