Department of Plant Science and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.
Plant Genomics and Breeding Institute, Seoul National University, Seoul 08826, Korea.
Int J Mol Sci. 2019 Jul 4;20(13):3287. doi: 10.3390/ijms20133287.
High-intensity ultraviolet-B (UV-B) irradiation is a complex abiotic stressor resulting in excessive light exposure, heat, and dehydration, thereby affecting crop yields. In the present study, we identified quantitative trait loci (QTLs) for resistance to high-intensity UV-B irradiation in soybean ( [L.]). We used a genotyping-by-sequencing approach using an F6 recombinant inbred line (RIL) population derived from a cross between Cheongja 3 (UV-B sensitive) and Buseok (UV-B resistant). We evaluated the degree of leaf damage by high-intensity UV-B radiation in the RIL population and identified four QTLs, , , , and , for UV-B stress resistance, together explaining 20% of the observed phenotypic variation. The genomic regions containing and and their syntenic blocks included other known biotic and abiotic stress-related QTLs. The QTL with the highest logarithm of odds (LOD) score of 3.76 was on Chromosome 12, containing two genes encoding spectrin beta chain, brain (SPTBN, Glyma.12g088600) and bZIP transcription factor21/TGACG motif-binding 9 (bZIP TF21/TGA9, Glyma.12g088700). Their amino acid sequences did not differ between the mapping parents, but both genes were significantly upregulated by UV-B stress in Buseok but not in Cheongja 3. Among five genes in on Chromosome 6, Glyma.06g319700 (encoding a leucine-rich repeat family protein) had two nonsynonymous single nucleotide polymorphisms differentiating the parental lines. Our findings offer powerful genetic resources for efficient and precise breeding programs aimed at developing resistant soybean cultivars to multiple stresses. Furthermore, functional validation of the candidate genes will improve our understanding of UV-B stress defense mechanisms.
高强度紫外线-B（UV-B）辐射是一种复杂的非生物胁迫因素，会导致过度光照、热量和脱水，从而影响作物产量。本研究中，我们鉴定了大豆对高强度 UV-B 辐射的抗性的数量性状位点（QTLs）。我们使用 Cheongja 3（UV-B 敏感）和 Buseok（UV-B 抗性）杂交产生的 F6 重组自交系（RIL）群体，通过测序进行基因型分析。我们评估了 RIL 群体中高强度 UV-B 辐射引起的叶片损伤程度，并鉴定了 4 个与 UV-B 胁迫抗性相关的 QTL，分别是 、 、 、和 ，共解释了 20%的表型变异。包含 和 的基因组区域及其同源框包含其他已知的生物和非生物胁迫相关的 QTL。LOD 得分最高（3.76）的 QTL 位于第 12 号染色体上，包含两个编码 spectrin beta 链、脑（SPTBN，Glyma.12g088600）和 bZIP 转录因子 21/TGACG 基序结合 9（bZIP TF21/TGA9，Glyma.12g088700）的基因。这两个基因在作图亲本之间的氨基酸序列没有差异，但在 Buseok 中，这两个基因在 UV-B 胁迫下均显著上调，而在 Cheongja 3 中则没有。第 6 号染色体上的 5 个基因（Glyma.06g319700，编码富含亮氨酸重复家族蛋白）在区分亲本系的两个基因中存在两个非同义单核苷酸多态性。我们的研究结果为开发具有多种抗性的大豆品种提供了有效的遗传资源，也为提高大豆对高强度 UV-B 辐射的抗性提供了理论依据。此外，候选基因的功能验证将提高我们对 UV-B 胁迫防御机制的理解。
