Zhang Dan, Li Hongyan, Wang Jinshe, Zhang Hengyou, Hu Zhenbin, Chu Shanshan, Lv Haiyan, Yu Deyue
Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University Zhengzhou, China.
Zhengzhou National Subcenter for Soybean Improvement/Key Laboratory of Oil Crops in Huanghuaihai Plains, Institute of Industrial Crops, Henan Academy of Agricultural Sciences Zhengzhou, China.
Front Plant Sci. 2016 Mar 30;7:372. doi: 10.3389/fpls.2016.00372. eCollection 2016.
Phosphorus (P) is essential for all living cells and organisms, and low-P stress represents a major constraint on plant growth and yield worldwide. Soybean is an important economical resource of protein and oil for human and animals, and soybean is also a high-P demand species that is sensitive to low-P stress, which is considered a major constraint on soybean production. However, P efficiency is an important complex quantitative trait involving multiple genes, and the mechanisms underlying soybean P efficiency are largely unknown. Here, we reported the construction of a high-density genetic map using a specific-locus amplified fragment sequencing (SLAF-seq) strategy in soybean. This map, spanning 3020.59 cM in length, contained 6159 markers on 20 chromosomes, with an average distance of 0.49 cM between adjacent markers. Based on this map, 20 loci, including eight novel loci, associated with P efficiency-related traits were identified across multiple years and treatments. The confidence intervals of almost all QTLs were refined significantly, and the accuracy of this map was evidenced by coincident detections of the previously identified P efficiency-related genes GmACP1 and GmPT1. Notably, a highly significant novel QTL located on chromosome 4, q4-2, was identified across traits, years and treatments. Several candidate genes, such as a pectin methylesterase-encoding gene (Glyma.04G214000) and a protein kinase gene (Glyma.13G161900), with significantly differential expression upon low-P stress were considered as promising candidates involved in regulating soybean P efficiency. Markers that tightly associated with P efficiency could be used for marker-assisted selection in a soybean P efficient breeding program. Further, dissection of these QTLs will facilitate gene cloning underlying P efficiency in soybean, and increase our understanding of efficient use of P in enhancing crop yield.
磷（P）对所有活细胞和生物体都至关重要，低磷胁迫是全球范围内植物生长和产量的主要限制因素。大豆是人类和动物重要的蛋白质和油脂经济资源，并且大豆也是对磷需求量大且对低磷胁迫敏感的物种，低磷胁迫被认为是大豆生产的主要限制因素。然而，磷效率是一个涉及多个基因的重要复杂数量性状，大豆磷效率的潜在机制在很大程度上尚不清楚。在此，我们报道了利用特异位点扩增片段测序（SLAF-seq）策略构建大豆高密度遗传图谱。该图谱全长3020.59厘摩，包含20条染色体上的6159个标记，相邻标记间的平均距离为0.49厘摩。基于此图谱，在多年和多种处理条件下鉴定出20个与磷效率相关性状相关的位点，包括8个新位点。几乎所有数量性状基因座的置信区间都得到了显著优化，先前鉴定的磷效率相关基因GmACP1和GmPT1的重合检测证明了该图谱的准确性。值得注意的是，在多个性状、年份和处理条件下均鉴定出一个位于4号染色体上的高度显著的新数量性状基因座q4-2。几个在低磷胁迫下表达有显著差异的候选基因，如一个编码果胶甲酯酶的基因（Glyma.04G214000）和一个蛋白激酶基因（Glyma.13G161900），被认为是参与调控大豆磷效率的有前景的候选基因。与磷效率紧密相关的标记可用于大豆磷高效育种计划中的标记辅助选择。此外，对这些数量性状基因座的剖析将有助于克隆大豆磷效率相关基因，并增进我们对通过高效利用磷来提高作物产量的理解。
