Co-expression of cellulase and xylanase genes in toward enhanced bioethanol production from corn stover.

Lignocellulose is considered as a good resource for producing renewable energy. Previous studies have shown the synergistic action between cellulase and xylanase during lignocellulose biohydrolysis. In order to achieve the same effect in to enhance the practical biotransformation, two recombinant strains (-CBH-CA and -CBH-TS) with co-expressed cellulase and xylanase were constructed. The cellulase and xylanase activities in -CBH-CA and -CBH-TS were 716.43 U/mL and 205.13 U/mL, 931.27 U/mL and 413.70 U/mL, respectively. The recombinant can use the partly delignified corn stover (PDCS) more efficiently and more ethanol producted than only expressing cellulase. Fermentation with -CBH-CA and -CBH-TS using PDCS ethanol yields increased by 1.7 and 2.1 folds higher than -CBH, 2.8 and 3.4 folds higher than the wild type . The strategy of co-expression cellulase and xylanase in is effective and can be foundation to research the mechanism of synergy effect of cellulose and xylanase.