Cao Jun, Cheng Xiuzhu
Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
Animals (Basel). 2019 Oct 28;9(11):873. doi: 10.3390/ani9110873.
Cytochrome P450s (CYPs) are a family of membrane-bound mono-oxygenase proteins, which are involved in cell metabolism and detoxification of various xenobiotic substances. In this study, we identified 58 putative genes in Amur stickleback () based on the transcriptome sequencing. Conserved motif distribution suggested their functional relevance within each group. Some present recombination events have accelerated the evolution of this gene family. Moreover, a few positive selection sites were identified, which may have accelerated the functional divergence of this family of proteins. Expression patterns of these genes were investigated and indicated that most were affected by dimethoate treatment, suggesting that were involved in the detoxication of dimethoate. This study will provide a foundation for the further functional investigation of genes in fishes.
细胞色素P450（CYPs）是一类膜结合单加氧酶蛋白家族，参与细胞代谢以及各种外源性物质的解毒过程。在本研究中，我们基于转录组测序在黑龙江刺鱼（）中鉴定出58个推定基因。保守基序分布表明了它们在每组中的功能相关性。一些现存的重组事件加速了该基因家族的进化。此外，还鉴定出了一些正选择位点，这些位点可能加速了该蛋白家族的功能分化。对这些基因的表达模式进行了研究，结果表明大多数基因受乐果处理的影响，这表明它们参与了乐果的解毒过程。本研究将为进一步开展鱼类基因的功能研究奠定基础。
