Transcriptome-Based Identification and Molecular Evolution of the Genes and Expression Profiling under Dimethoate Treatment in Amur Stickleback ().

Cytochrome P450s (CYPs) are a family of membrane-bound mono-oxygenase proteins, which are involved in cell metabolism and detoxification of various xenobiotic substances. In this study, we identified 58 putative genes in Amur stickleback () based on the transcriptome sequencing. Conserved motif distribution suggested their functional relevance within each group. Some present recombination events have accelerated the evolution of this gene family. Moreover, a few positive selection sites were identified, which may have accelerated the functional divergence of this family of proteins. Expression patterns of these genes were investigated and indicated that most were affected by dimethoate treatment, suggesting that were involved in the detoxication of dimethoate. This study will provide a foundation for the further functional investigation of genes in fishes.