Institute of Life Sciences, Jiangsu University, Zhenjiang, 212013, China.
Institute of Life Sciences, Jiangsu University, Zhenjiang, 212013, China.
Fish Shellfish Immunol. 2018 Nov;82:432-441. doi: 10.1016/j.fsi.2018.08.048. Epub 2018 Aug 23.
Tetraspanins are a family of membrane proteins, which play important roles in many aspects of cell biology and physiology via binding other tetraspanins or proteins. In this study, we identified 251 putative tetraspanin genes in 6 teleost fishes. Conserved gene organization and motif distribution suggested their functional relevance existing in each group. Synteny analyses implied conserved and dynamic evolution characteristics of this gene family in several vertebrates. We also found that some recombination events have accelerated the evolution of this gene family. Moreover, a few positive selection sites were identified. Expression patterns of some tetraspanins were further studied under organophosphorus stress using transcriptome sequencing. Functional network analyses identified some interacting genes that exhibited 174 interactions, which reflected the diversity of tetraspanin binding proteins. The results will provide a foundation for the further functional investigation of the tetraspanin genes in fishes.
四跨膜蛋白是一类膜蛋白，通过与其他四跨膜蛋白或蛋白结合，在细胞生物学和生理学的许多方面发挥重要作用。在这项研究中，我们在 6 种硬骨鱼中鉴定出了 251 个推定的四跨膜蛋白基因。保守的基因组织和基序分布表明它们在每个组中都具有功能相关性。同线性分析表明，该基因家族在几种脊椎动物中具有保守和动态的进化特征。我们还发现，一些重组事件加速了这个基因家族的进化。此外，还鉴定出了一些正选择位点。我们还通过转录组测序研究了一些四跨膜蛋白在有机磷胁迫下的表达模式。功能网络分析确定了一些相互作用的基因，这些基因表现出 174 种相互作用，反映了四跨膜蛋白结合蛋白的多样性。这些结果将为鱼类四跨膜蛋白基因的进一步功能研究提供基础。
