Establishment and biological properties of mouse granulocytic leukemic cell line-L833.

A granulocytic leukemic cell line, called L833 has been established through culturing in vitro from mouse bone marrow of transplantable granulocytic leukemia. More than 280 passages have been performed in 3 years. Cell growth has been rapid and stable. Incidence of tumour formation was 100% with various routes of inoculation. The nature of granulocytic leukemia was confirmed by examination of cytology, cytochemistry, pathology and ultrastructural changes. Analysis proved chromosomes to be hypodiploid with model number of 39, loss of chromosomes, and presence of a marker. Besides the chromosomal change as mentioned above, both L883-A and L833-B derived from colonies formed from the L833 cells cultured in semi-solid agar medium, have their own marker. The cell line was sensitive to various types of antitumour agents in varying degrees. It was also sensitive to ionizing radiation. D0 values of L833 and L883-A cells were 98.8 and 104.9 rad, respectively. The results were similar to that of L801. Establishment of this cell line is of important significance for studying leukemia and screening anti-tumour agents, as well as provides a useful direct, economic and precisely quantitative tool for other relative studies.