一种辐射诱导的犬单核细胞白血病细胞系（RK9ML-1）的建立及部分特性研究

Establishment and partial characterization of a radiation-induced canine monocytic leukemic cell line (RK9ML-1).

作者信息

Kawakami T, Cain G, Taylor N

机构信息

University of California, Davis Laboratory for Energy-Related Research 95616.

出版信息

Leuk Res. 1989;13(8):709-14. doi: 10.1016/0145-2126(89)90060-x.

DOI:10.1016/0145-2126(89)90060-x

PMID:2796377

Abstract

A myeloid leukemic cell line, designated RK9ML-1, was established from a dog with acute radiation-induced monocytic leukemia. Based on cytochemical stains which reacted positively only with nonspecific esterase, morphological and ultrastructural characteristics which indicated the presence of phagocytic vacuoles and lysosomal bodies, and cell surface properties which indicated the presence of Fc receptors, all the findings support that RK9ML-1 is of monocytic lineage. Chromosomal analysis of the cell line indicated the cells to be hypodiploid with acrocentric autosomes characteristic of canine cells.

摘要

一种名为RK9ML-1的髓系白血病细胞系，是从一只患有急性辐射诱导单核细胞白血病的犬中建立的。基于仅与非特异性酯酶呈阳性反应的细胞化学染色、表明存在吞噬泡和溶酶体的形态学和超微结构特征以及表明存在Fc受体的细胞表面特性，所有这些发现都支持RK9ML-1属于单核细胞系。对该细胞系的染色体分析表明，这些细胞为亚二倍体，具有犬类细胞特有的近端着丝粒常染色体。