在LACA小鼠中建立可移植性髓细胞白血病（L801）模型。

Establishment of a model of transplantable myelocytic leukemia (L801) in LACA mice.

作者信息

Zhao N K, Du W X, Liu X M, Li X L, Gao F M, Ji X J, Zhang F R, Liu Y, Li K

出版信息

Sci Sin B. 1985 Jul;28(7):736-44.

Abstract

A transplantable myelocytic leukemia model of LACA mice, designated by the name of L801, was established by intravenous injection of spleen cell suspension from mice with radiation-induced myelocytic leukemia into mice of the same strain. Until now, for more than three years, the L801 has maintained stable and rapid growth and has been reproduced for over 130 serial passages. The incidence of leukemia in inoculated animals was approximately 100% and mean survival time was 10.9 +/- 2.1 days. The L801 is of myelocytic type which has been determined by cytological, cytochemical, pathological and ultrastructural observations. Its karyotype was hypodiploid, characterized by modal number of 39, loss of Y chromosome and an abnormal huge marker chromosome. The cell cycle duration of the L801 was 16 h. C-type viral particles were observed under the electron-microscope. The L801 was sensitive, to varying extents, to various anti-tumor agents. We presume that the L801 is a useful tool in studies on mechanism of leukemogenesis, anti-tumor agent screening and treatment of experimental tumors.

摘要

通过将辐射诱导的髓细胞白血病小鼠的脾细胞悬液静脉注射到同品系小鼠体内，建立了一种名为L801的可移植性LACA小鼠髓细胞白血病模型。到目前为止，三年多来，L801一直保持稳定快速生长，已连续传代130多次。接种动物的白血病发病率约为100%，平均存活时间为10.9±2.1天。通过细胞学、细胞化学、病理学和超微结构观察确定L801为髓细胞类型。其核型为亚二倍体，特征是众数为39，Y染色体缺失和一条异常的巨大标记染色体。L801的细胞周期持续时间为16小时。在电子显微镜下观察到C型病毒颗粒。L801对各种抗肿瘤药物有不同程度的敏感性。我们推测L801是白血病发生机制研究、抗肿瘤药物筛选和实验性肿瘤治疗的有用工具。