Department of Pathology, Gyeongsang National University Changwon Hospital, Changwon, Republic of Korea.
Department of Pathology, Gyeongsang National University Changwon Hospital, Changwon, Republic of Korea
In Vivo. 2019 Nov-Dec;33(6):1885-1892. doi: 10.21873/invivo.11682.
There have been many attempts to predict the prognosis of lung cancer based on the expression patterns of P16 protein, but with limited success. The Eighth American Joint Committee on Cancer (AJCC) for head and neck cancer recently developed new criteria for evaluating P16 expression. Here, we applied these new criteria to evaluate the prognosis of non-small cell lung cancer (NSCLC).
A total of 148 patients who had undergone surgery for NSCLC were enrolled in the study. P16 protein expression patterns from NSCLC tissue microarray samples were examined by immunohistochemical analysis. The Eighth AJCC head and neck cancer staging criteria were used to evaluate positive P16 expression (moderate/strong nuclear expression intensity and distribution >75% cells) in NSCLC. The relationship between P16 expression and clinicopathological factors were evaluated and survival analysis was included.
Negative P16 expression was significantly associated with NSCLC with lymph node metastasis (p=0.025). In addition, patients with NSCLC with negative P16 expression demonstrated poor disease-free and disease-specific survival in multivariate analysis. The Kaplan-Meier survival curve confirmed that negative P16 expression was significantly correlated with a poor disease-free survival (p=0.017) and disease-specific survival (p=0.016).
P16 expression defined with the new AJCC criteria is useful for detecting lymph node metastasis in NSCLC.
已有许多尝试基于 P16 蛋白的表达模式来预测肺癌的预后，但成功率有限。第八版美国癌症联合委员会（AJCC）最近制定了新的头颈部癌症 P16 表达评估标准。在此，我们应用这些新标准来评估非小细胞肺癌（NSCLC）的预后。
本研究共纳入 148 例接受 NSCLC 手术的患者。通过免疫组织化学分析检测 NSCLC 组织微阵列样本中的 P16 蛋白表达模式。应用第八版 AJCC 头颈部癌症分期标准评估 NSCLC 中 P16 阳性表达（中度/强核表达强度和分布>75%细胞）。评估 P16 表达与临床病理因素的关系，并进行生存分析。
P16 阴性表达与 NSCLC 淋巴结转移显著相关（p=0.025）。此外，多因素分析显示 P16 阴性表达的 NSCLC 患者无病生存和疾病特异性生存较差。Kaplan-Meier 生存曲线证实 P16 阴性表达与无病生存（p=0.017）和疾病特异性生存（p=0.016）显著相关。
应用 AJCC 新标准定义的 P16 表达可用于检测 NSCLC 中的淋巴结转移。
