Júnior F A Da Silva, Silva C Monteiro Da, Almeida F B De, Rodrigues-Silva R
Faculdade de Ciências Biológicas e da Saúde - Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM). Rod. MGT 367, 5000 - Alto da Jacuba, Diamantina - MG, 39100-000, Brazil.
Laboratório de Helmintos Parasitos de Vertebrados - LHPV, Instituto Oswaldo Cruz - IOC, Fundação Oswaldo Cruz - FIOCRUZ, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, RJ, 21040-360, Brazil.
Helminthologia. 2018 Jul 28;55(3):204-212. doi: 10.2478/helm-2018-0014. eCollection 2018 Sep.
This study was carried out to estimate the minimal number of eggs present in adult uterus. Samples were collected during post-mortem inspection and were submitted to light microscopy (bright field). The length, width, the total area of the parasite, uterus, and eggs were measured. The ImageJ software was used to calculate the area of the different parameters analyzed in this study. It was possible to observe that the uterus corresponds on average to 51.9 % of the total area of the parasite (ranging from 45 to 64 %). The number of eggs present in the uterus of parasites ranged from 5,946 to 15,813. To estimate the number of eggs three scenarios were considered, where the first taken into account the number of whole eggs observed in the image. In the second way to estimate the number of eggs, all the structures were considered (whole eggs and fractions that could be delimited) and compared with manual counting. Finally, in the last scenario, was considered an occupancy rate of 100 % of the uterine area per eggs, since there are overlapping eggs and these cannot be correctly delimited and accounted for. This study describes an important tool for quantifying eggs in a nondestructive manner and aggregate information until then is not explained by other works.
本研究旨在估计成虫子宫内存在的最小卵数。样本在尸检期间采集，并提交至光学显微镜（明场）观察。测量了寄生虫、子宫和卵的长度、宽度及总面积。使用ImageJ软件计算本研究中分析的不同参数的面积。可以观察到，子宫平均占寄生虫总面积的51.9%（范围为45%至64%）。寄生虫子宫内的卵数在5946至15813个之间。为了估计卵数，考虑了三种情况，第一种情况是考虑图像中观察到的完整卵的数量。第二种估计卵数的方法是考虑所有结构（完整卵和可界定的部分）并与人工计数进行比较。最后，在最后一种情况下，考虑每个卵对子宫区域的占有率为100%，因为存在重叠的卵，无法正确界定和计数。本研究描述了一种以非破坏性方式量化卵的重要工具，此前其他研究未解释的汇总信息。
