Liu Wei, Liu Heqi, Zhang Zongde, Huang Jiapeng
Department of Anesthesiology, Beijing Chest Hospital, Capital Medical University, Beijing, China.
Department of Anesthesiology, Beijing Children's Hospital, Capital Medical University, Beijing, China.
Dose Response. 2019 Oct 14;17(4):1559325819882873. doi: 10.1177/1559325819882873. eCollection 2019 Oct-Dec.
This study compared analgesic effects and μ-opioid receptor expression levels during long-term intraperitoneal and intrathecal treatment in a bone cancer pain rat.
Twenty-four female Sprague-Dawley rats were injected Walker 256 tumor cells into the femur to create a bone cancer pain model. The control group was injected with saline intraperitoneally and intrathecally. The intraperitoneal group was injected with morphine intraperitoneally and saline intrathecally. The intrathecal group was injected saline intraperitoneally and morphine intrathecally. Changes in pain threshold, μ-opioid receptor expression levels in spinal cord, and tumor tissue were compared between 3 groups.
The intrathecal morphine group and the intraperitoneal group showed no difference in analgesia effects ( > .05). Western blot and immunohistochemical staining of μ-opioid receptors demonstrated that its level in the intrathecal group was significantly lower than the intraperitoneal group ( < .05) and without significant difference with the control group ( > .05). The expression levels of μ-opioid receptor in the spinal cord tissue did not reveal a difference among these 3 groups ( > .05).
Intrathecal group and intraperitoneal group showed significant difference in μ-opioid receptor expressions although with no difference in analgesia effects. Long-term intrathecal morphine administration provided similar analgesia compared to systemic morphine.
本研究比较了骨癌痛大鼠长期腹腔内和鞘内给药时的镇痛效果及μ-阿片受体表达水平。
将24只雌性Sprague-Dawley大鼠股骨内注射Walker 256肿瘤细胞以建立骨癌痛模型。对照组腹腔内和鞘内注射生理盐水。腹腔内给药组腹腔内注射吗啡，鞘内注射生理盐水。鞘内给药组腹腔内注射生理盐水，鞘内注射吗啡。比较三组之间痛阈、脊髓和肿瘤组织中μ-阿片受体表达水平的变化。
鞘内吗啡组和腹腔内给药组镇痛效果无差异（>0.05）。μ-阿片受体的蛋白质免疫印迹法和免疫组织化学染色显示，鞘内给药组其水平显著低于腹腔内给药组（<0.05），与对照组无显著差异（>0.05）。脊髓组织中μ-阿片受体表达水平在这三组之间无差异（>0.05）。
鞘内给药组和腹腔内给药组μ-阿片受体表达存在显著差异，尽管镇痛效果无差异。与全身应用吗啡相比，长期鞘内注射吗啡提供了相似的镇痛效果。
