Lee HansongI, Yeom Miji, Shin Seoungwoo, Jeon Kyungeun, Park Deokhoon, Jung Eunsun
Biospectrum Life Science Institute, A-1805, U-Tower, 767, Sinsu-ro, Suji-gu, 16827 Yongin-si, Gyeonggi-do, Republic of Korea.
Evid Based Complement Alternat Med. 2019 Sep 22;2019:5045491. doi: 10.1155/2019/5045491. eCollection 2019.
is an aromatic herb that has a wide range of biological activities, including antimicrobial, antifungal, anti-inflammatory, and hepatoprotective properties. Although there are a few reports on the antioxidant property of , its cytoprotective activity against oxidative stress has not been reported yet. The objective of this study was to determine the protective activity of aqueous extract (MSAE) against hydrogen peroxide- (HO-) induced oxidative stress and apoptosis in human keratinocyte HaCaT cells. MSAE pretreatment decreased HO-induced cytotoxicity and suppressed HO-induced intracellular ROS generation. Furthermore, MSAE suppressed expression levels of HO-induced apoptotic genes such as cleaved caspase-3, caspase-9, and cleaved poly (ADP-ribose) polymerase (PARP). Pretreatment with MSAE induced expression of phase II enzyme such as HO-1 through translocation of NF-E2-related factor (Nrf2) upon HO exposure. These results revealed that the cytoprotective effect of MSAE against oxidative stress-induced cell death was associated with activation of Nrf2-mediated phase II enzyme expression.
是一种具有广泛生物活性的芳香草本植物，包括抗菌、抗真菌、抗炎和保肝特性。尽管有一些关于其抗氧化特性的报道，但其对氧化应激的细胞保护活性尚未见报道。本研究的目的是确定水提取物（MSAE）对过氧化氢（H₂O₂）诱导的人角质形成细胞HaCaT细胞氧化应激和凋亡的保护活性。MSAE预处理降低了H₂O₂诱导的细胞毒性，并抑制了H₂O₂诱导的细胞内活性氧生成。此外，MSAE抑制了H₂O₂诱导的凋亡基因如裂解的半胱天冬酶-3、半胱天冬酶-9和裂解的聚（ADP-核糖）聚合酶（PARP）的表达水平。在H₂O₂暴露时，通过NF-E2相关因子（Nrf2）的易位，MSAE预处理诱导了II期酶如HO-1的表达。这些结果表明，MSAE对氧化应激诱导的细胞死亡的细胞保护作用与Nrf2介导的II期酶表达的激活有关。
