College of Life Sciences, Qufu Normal University, Qufu, Shandong 273165, China.
Research Institute of Experimental Detection, Xinjiang Oilfield Company, Petro China, Karamay, Xinjiang 834000, China.
Biomed Res Int. 2019 Oct 2;2019:2193453. doi: 10.1155/2019/2193453. eCollection 2019.
With the development of molecular ecology, increasing low-abundance microbial populations were detected in oil reservoirs. However, our knowledge about the oil recovery potential of these populations is lacking. In this study, the oil recovery potential of low-abundance that accounts for less than 0.5% in microbial communities of a water-flooding oil reservoir was investigated. On the one hand, sp. strain ZQ-4 was isolated from the water-flooding reservoir, and the oil recovery potential was evaluated from the perspective of metabolisms and oil-displacing test. On the other hand, the strain has alkane hydroxylase genes and P450 CYP153 and can degrade hydrocarbons and produce surfactants. The core-flooding test indicated that displacing fluid with 2% ZQ-4 fermentation broth increased 18.82% oil displacement efficiency, and in situ fermentation of ZQ-4 increased 1.97% oil displacement efficiency. Furthermore, the responses of in the reservoir accompanied by the nutrient stimulation process was investigated and showed that in some oil production wells significantly increased in the initial phase of nutrient injection and sharply decreased along with the continuous nutrient injection. Overall, this study indicates that sp. strain has application potential for enhancing oil recovery through an ex situ way, yet the ability of oil recovery in situ based on nutrient injection is limited.
随着分子生态学的发展，越来越多的低丰度微生物种群在油藏中被检测到。然而，我们对这些种群的采油潜力知之甚少。在这项研究中，我们调查了在注水油藏微生物群落中丰度低于 0.5%的低丰度微生物的采油潜力。一方面，从注水油藏中分离到了一株 sp. 菌株 ZQ-4，并从代谢和驱油试验的角度评估了其采油潜力。另一方面，该菌株具有烷烃羟化酶基因和 P450 CYP153，能够降解烃类并产生表面活性剂。岩心驱替试验表明，用 2%ZQ-4 发酵液驱替流体可提高 18.82%的驱油效率，原位发酵 ZQ-4 可提高 1.97%的驱油效率。此外，还研究了在营养刺激过程中伴随的 响应，结果表明，在营养注入的初始阶段，一些采油井中的 显著增加，随着营养的持续注入，其数量急剧下降。总的来说，这项研究表明，sp. 菌株通过异位方式提高采油效率具有应用潜力，但基于营养注入的原位采油能力有限。
