CAS Key Laboratory of Urban Pollutant Conversion, Department of Applied Chemistry , University of Science and Technology of China , Hefei 230026 , China.
Environ Sci Technol. 2019 Nov 19;53(22):13374-13381. doi: 10.1021/acs.est.9b05250. Epub 2019 Oct 30.
An increasing amount of attention has been given to antimicrobial resistance in the environment because of its substantial threat to human health. The effluent from municipal wastewater treatment plants has been regarded as one of the important sources for the spread of antibiotic resistance genes (ARGs). However, conventional disinfection techniques fail to effectively remove ARGs from effluents. In this work, in situ synthesized hydrated manganese oxide (HMO) coupled with permanganate was applied for the first time in ARG removal from the effluent of wastewater treatment plants. The results show that five ARGs (I, II, Q, O, and W) as well as the I1 and 16S rRNA genes had removal efficiencies of 2.46-4.23 logs, which were significantly higher than those obtained by using these reagents individually. This implied that there was a synergistic effect between permanganate and HMO toward the removal of ARGs. Moreover, the contributions of HMO coagulation and permanganate oxidation to ARG removal were semiquantitatively studied, which demonstrated that destruction of the microbial cells by oxidation and removal of the extracellular ARGs released by coagulation were the two main processes in this system. The results of this study provide an alternative method for ARG removal from the effluent of wastewater treatment plants with high efficiencies to control the spreading of ARGs.
由于其对人类健康的重大威胁，环境中抗生素耐药性越来越受到关注。城市污水处理厂的污水被认为是抗生素耐药基因（ARGs）传播的重要来源之一。然而，传统的消毒技术无法有效地从污水中去除 ARGs。在这项工作中，首次将原位合成的水合氧化锰（HMO）与高锰酸盐结合应用于去除污水处理厂污水中的 ARGs。结果表明，五种 ARGs（I、II、Q、O 和 W）以及 I1 和 16S rRNA 基因的去除效率为 2.46-4.23 个对数，明显高于单独使用这些试剂时的去除效率。这表明高锰酸盐和 HMO 之间存在协同去除 ARGs 的作用。此外，还对半定量研究了 HMO 混凝和高锰酸盐氧化对 ARG 去除的贡献，结果表明，氧化破坏微生物细胞和混凝去除释放的胞外 ARGs 是该体系中的两个主要过程。本研究为高效去除污水处理厂污水中的 ARGs 提供了一种替代方法，以控制 ARGs 的传播。
