Centre for Biotechnology, Siksha O Anusandhan University, Bhubaneswar, Odisha, India.
Centre for Enzyme Innovation, School of Biological Sciences, University of Portsmouth, Portsmouth, UK.
Prep Biochem Biotechnol. 2020;50(6):578-584. doi: 10.1080/10826068.2020.1719513. Epub 2020 Feb 3.
Lipase based formulations has been a rising interest to laundry detergent industry for their eco-friendly property over phosphate-based counterparts and compatibility with chemical detergents ingredients. A thermo-stable sp. ARS-1 isolated from Taptapani Hotspring, India was characterized for optimum lipase production employing statistical model central composite design (CCD) under four independent variables (temperature, pH, % moisture and bio-surfactant) by solid substrate fermentation (SSF) using mustard cake. The output was utilized to find the effect of parameters and their interaction employing response surface methodology (RSM). A quadratic regression with = 0.955 established the model to be statically best fitting and a predicted highest lipase production of 29.4 IU/g at an optimum temperature of 57.5 °C, pH 8.31, moisture 50% and 1.2 mg of bio-surfactant. Experimental production of 30.3 IU/g lipase at above conditions validated the fitness of model. ARS-1 produced lipase was found to resist almost all chemical detergents as well as common laundry detergent, proving it to be a prospective additive for incorporation.
基于脂肪酶的配方因其具有环保性能，优于含磷的配方，并且与化学洗涤剂成分兼容，因此引起了洗衣洗涤剂行业的关注。从印度 Taptapani 温泉中分离出的一株耐热性 sp. ARS-1，通过固态发酵（SSF）利用芥菜饼，采用统计模型中心复合设计（CCD）在四个独立变量（温度、pH 值、水分百分比和生物表面活性剂）下进行了最佳脂肪酶生产的特性研究。利用响应面法（RSM）对参数及其相互作用的影响进行了输出分析。二次回归方程 = 0.955 表明该模型是静态最佳拟合，预测在最佳温度 57.5°C、pH 值 8.31、水分 50%和 1.2mg 生物表面活性剂的条件下，脂肪酶的最高产量为 29.4IU/g。在上述条件下实验生产的 30.3IU/g 脂肪酶验证了模型的拟合度。发现 ARS-1 产生的脂肪酶几乎可以抵抗所有化学洗涤剂和普通洗衣洗涤剂，证明它是一种有前景的添加物。
