微生物脂肪酶的生产策略及生物技术相关性：综述
Production strategies and biotechnological relevance of microbial lipases: a review.
机构信息
Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal (Westville campus), Private Bag X54001, Durban, 4000, Republic of South Africa.
出版信息
Braz J Microbiol. 2021 Sep;52(3):1257-1269. doi: 10.1007/s42770-021-00503-5. Epub 2021 Apr 27.
Abstract
Lipases are enzymes that catalyze the breakdown of lipids into long-chain fatty acids and glycerol in oil-water interface. In addition, they catalyze broad spectrum of bioconversion reactions including esterification, inter-esterification, among others in non-aqueous and micro-aqueous milieu. Lipases are universally produced from plants, animals, and microorganisms. However, lipases from microbial origin are mostly preferred owing to their lower production costs, ease of genetic manipulation etc. The secretion of these biocatalysts by microorganisms is influenced by nutritional and physicochemical parameters. Optimization of the bioprocess parameters enhanced lipase production. In addition, microbial lipases have gained intensified attention for a wide range of applications in food, detergent, and cosmetics industries as well as in environmental bioremediation. This review provides insights into strategies for production of microbial lipases for potential biotechnological applications.
摘要
脂肪酶是一种在油水界面上将脂肪分解为长链脂肪酸和甘油的酶。此外，它们还能催化酯酯化、酯交换等多种生物转化反应，包括非水相和微水相。脂肪酶普遍存在于植物、动物和微生物中。然而，由于微生物来源的脂肪酶生产成本更低、遗传操作更容易等原因，因此大多优先选择微生物来源的脂肪酶。微生物生物催化剂的分泌受营养和理化参数的影响。优化生物过程参数可提高脂肪酶的产量。此外，微生物脂肪酶在食品、洗涤剂和化妆品行业以及环境生物修复等领域的广泛应用也引起了人们的强烈关注。本文综述了微生物脂肪酶生产的策略，以期为其在潜在生物技术中的应用提供参考。
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