Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan, 430079, PR China.
China Resources & Wisco General Hospital, Wuhan, 430080, PR China.
Biosens Bioelectron. 2020 Jan 15;148:111780. doi: 10.1016/j.bios.2019.111780. Epub 2019 Oct 11.
Estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor-2 (HER2) are the three crucial biomarkers for the clinical diagnosis of breast cancer. Sensitive and precise detection of ER, PR, and HER2 is of great significance for the diagnosis of breast cancer. Herein, through a simple solvent-induced self-assembly process, the self-carried allochroic nanoparticles were prepared by using some hydrophobic pH indicator molecules for allochroic NPs-linked immunosorbent assay (named ALISA) of ER, PR, and HER2, respectively. Meanwhile, the introduction of bovine serum albumin (BSA) and antibody (Ab) enhanced the dispersity of the self-assembled nanoparticles as signal tags. Since the ultra-high loading and high-efficiency release of pH indicators, the ALISA exhibitssatisfactory selectivity and sensitivity, which demonstrated the great potential in the early diagnosis and postoperative monitoring of breast cancers. Furthermore, the smartphone was introduced to combine with the ALISA for point-of-care testing, indicating the high feasibility in practical applications.
雌激素受体 (ER)、孕激素受体 (PR) 和人表皮生长因子受体-2 (HER2) 是乳腺癌临床诊断的三个关键生物标志物。敏感、准确地检测 ER、PR 和 HER2 对乳腺癌的诊断具有重要意义。在此，通过简单的溶剂诱导自组装过程，使用一些疏水性 pH 指示剂分子制备了自携变色纳米粒子，分别用于 ER、PR 和 HER2 的异相 NPs-酶联免疫吸附测定 (ALISA)。同时，牛血清白蛋白 (BSA) 和抗体 (Ab) 的引入增强了自组装纳米粒子作为信号标记物的分散性。由于 pH 指示剂的超高负载量和高效释放，ALISA 表现出令人满意的选择性和灵敏度，这表明其在乳腺癌的早期诊断和术后监测方面具有巨大的潜力。此外，引入智能手机与 ALISA 相结合进行即时检测，表明其在实际应用中具有很高的可行性。
