用于全进化枝系统发育研究的叶绿体引物。 （你提供的原文似乎不完整，后面应该还有具体所研究的对象等内容）
Chloroplast primers for clade-wide phylogenetic studies of .
作者信息
Morales-Briones Diego F, Arias Tatiana, Di Stilio Verónica S, Tank David C
机构信息
Department of Biological Sciences University of Idaho 875 Perimeter Dr. MS 3051 Moscow Idaho 83844-3051 USA.
Stillinger Herbarium University of Idaho 875 Perimeter Dr. MS 3026 Moscow Idaho 83844-3026 USA.
出版信息
Appl Plant Sci. 2019 Oct 16;7(10):e11294. doi: 10.1002/aps3.11294. eCollection 2019 Oct.
PREMISE
Chloroplast primers were developed for phylogenetic and comparative studies in (Ranunculaceae).
METHODS AND RESULTS
We assembled and annotated the complete plastome sequence of by combining multiple whole genome sequencing libraries. Using transcriptome-sequencing libraries, we also assembled a partial plastome of the related species . From the newly assembled plastomes and one previously sequenced plastome, we designed and validated 28 primer pairs to target variable portions of the chloroplast genome in . Furthermore, we tested the validated primers in 62 species of . The total alignment length of the 28 regions was 15,268 bp with 2443 variable sites and 92% character occupancy.
CONCLUSIONS
The newly developed chloroplast primer pairs improve the phylogenetic resolution (bootstrap support and tree certainty) in and will be a useful resource for future phylogenetic and evolutionary studies for species in the genus and in close relatives in Thalictroideae.
前提
已开发叶绿体引物用于毛茛科的系统发育和比较研究。
方法与结果
我们通过合并多个全基因组测序文库，组装并注释了唐松草的完整叶绿体基因组序列。利用转录组测序文库，我们还组装了相关物种东亚唐松草的部分叶绿体基因组。从新组装的叶绿体基因组和一个先前测序的叶绿体基因组中，我们设计并验证了28对引物，以靶向唐松草叶绿体基因组的可变区域。此外，我们在62种唐松草属植物中测试了经过验证的引物。这28个区域的总比对长度为15268 bp，有2443个可变位点，特征占有率为92%。
结论
新开发的叶绿体引物对提高了唐松草属的系统发育分辨率（自展支持率和树形确定性），并将成为该属及唐松草亚科近缘物种未来系统发育和进化研究的有用资源。
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