Goycoolea M V, Muchow D, Martinez G C, Aguila P B, Goycoolea H G, Goycoolea C V, Schachern P, Knight W
Minnesota Ear, Head, and Neck Clinic, University of Minnesota.
Arch Otolaryngol Head Neck Surg. 1988 Nov;114(11):1247-51. doi: 10.1001/archotol.1988.01860230041019.
An ultrastructural study was done in three sequential steps to determine if the human round-window membrane was permeable to macromolecules. Cationic ferritin was first placed for one hour in the round-window niche of two live rhesus monkeys. The same tracer was then placed in the same manner in two rhesus monkeys that had been dead for one hour. In both groups, cationic ferritin was observed to traverse the round-window membrane through pinocytotic vesicles into the scala tympani. After establishing that the transport capabilities of the round-window membrane of the monkey remained present one hour after death, cationic ferritin was placed for one hour in the round-window niche of two humans who had been dead for 30 minutes and one hour. The tracer was observed to traverse the round-window membrane through pinocytotic vesicles into the scala tympani in both humans. This report may be the first to document morphologically the permeability of human round-window membranes to macromolecules.
为确定人圆窗膜是否对大分子具有通透性，进行了三个连续步骤的超微结构研究。首先将阳离子铁蛋白放置在两只活恒河猴的圆窗龛中一小时。然后以相同方式将相同的示踪剂放置在两只已死亡一小时的恒河猴中。在两组中，均观察到阳离子铁蛋白通过胞饮小泡穿过圆窗膜进入鼓阶。在确定猴子圆窗膜的转运能力在死后一小时仍存在后，将阳离子铁蛋白放置在两名分别死亡30分钟和一小时的人的圆窗龛中一小时。在这两名受试者中均观察到示踪剂通过胞饮小泡穿过圆窗膜进入鼓阶。本报告可能是首个从形态学上证明人圆窗膜对大分子具有通透性的报告。
