Goycoolea M V, Muchow D, Schachern P
Minnesota Ear, Head and Neck Clinic, Minneapolis 55454.
Laryngoscope. 1988 Jun;98(6 Pt 2 Suppl 44):1-20. doi: 10.1288/00005537-198806001-00002.
Current research and an overall review of 12 years of round window membrane studies is presented. The approach, rationale, and concepts that have evolved from the studies are described. An ultrastructural study of the round window membrane of rhesus monkeys disclosed three basic layers: an outer epithelium, a middle core of connective tissue, and an inner epithelium. Morphologic evidence in monkeys, cats, and chinchillas suggests that these layers of the round window participate in absorption and secretion of substances to and from the inner ear, and that the entire membrane could play a role in the defense system of the ear. Cationic ferritin, horseradish peroxidase, 1-micron latex spheres, and neomycin-gold spheres placed in the middle ear of these experimental animals were observed to traverse the round window membrane through pinocytotic vesicles. Three-micron latex spheres and anionic ferritin were not incorporated by the membrane. Cationic ferritin and 1-micron latex spheres placed in perilymph were incorporated by the inner epithelial cells, suggesting absorptive capabilities of the round window membrane. Cationic ferritin was observed within the mesothelial cells underlying the scala tympani side of the basilar membrane, suggesting a role for these cells in the inner-ear defense system. A review of the subject and a general perspective from the author's viewpoint are discussed.
本文介绍了当前的研究以及对12年圆窗膜研究的全面综述。描述了从这些研究中演变而来的方法、基本原理和概念。对恒河猴圆窗膜的超微结构研究揭示了三个基本层：外层上皮、中间的结缔组织核心和内层上皮。在猴子、猫和毛丝鼠身上的形态学证据表明，圆窗的这些层参与了内耳物质的吸收和分泌，并且整个膜可能在耳朵的防御系统中发挥作用。观察到放置在这些实验动物中耳的阳离子铁蛋白、辣根过氧化物酶、1微米乳胶球和新霉素-金球通过胞饮小泡穿过圆窗膜。3微米乳胶球和阴离子铁蛋白未被该膜摄取。放置在外淋巴中的阳离子铁蛋白和1微米乳胶球被内层上皮细胞摄取，表明圆窗膜具有吸收能力。在基底膜鼓阶侧下方的间皮细胞内观察到阳离子铁蛋白，表明这些细胞在内耳防御系统中发挥作用。本文还讨论了该主题的综述以及作者观点的总体视角。
