Laowtammathron Chuti, Srisook Pimonwan, Chingsuwanrote Pimjai, Jiamvoraphong Nittaya, Waeteekul Supaporn, Terbto Papussorn, U-Pratya Yaowalak, Lorthongpanich Chanchao, Issaragrisil Surapol
Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Road, Bangkoknoi, Bangkok 10700, Thailand.
Division of Medical Genetics, Department of Obstetrics & Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Stem Cell Res. 2019 Dec;41:101597. doi: 10.1016/j.scr.2019.101597. Epub 2019 Oct 19.
CD34 cells were isolated from mobilized peripheral blood of a healthy donor and reprogrammed by nucleofection with episomal plasmids carrying l-MYC, LIN28, OCT4, SOX2, KLF4, EBNA-1, and shRNA against p53. The obtained MUSIi012-A cell line maintained the pluripotent phenotype, the ability to differentiate into all three germ layers, and a normal karyotype.
从健康供体的动员外周血中分离出CD34细胞，并用携带l-MYC、LIN28、OCT4、SOX2、KLF4、EBNA-1和针对p53的短发夹RNA（shRNA）的游离质粒通过核转染进行重编程。所获得的MUSIi012-A细胞系维持了多能表型、分化为所有三个胚层的能力以及正常的核型。
