School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Food Chem. 2020 Mar 30;309:125680. doi: 10.1016/j.foodchem.2019.125680. Epub 2019 Oct 19.
In this work, to improve the availability of the recognition sites of molecularly imprinted polymers (MIPs), ordered macroporous molecularly imprinted polymers (OMMIPs) were facilely prepared by grafting a quercetin-MIPs layer on the pore walls of the ordered macroporous thiol group functionalized silica. The pore structures were characterized by FTIR, Raman, SEM, BET and TGA measurements. The results indicated that OMMIPs possessed a nanoscale polymer layer, a more regular macroporous structure and a greater porosity compared with the traditional bulk MIPs (TBMIPs). The polymer content of OMMIPs was about 49.7%. Kinetic and isothermal adsorption experiments indicated that OMMIPs exhibited higher affinity and selectivity towards quercetin than its structural analogues. Moreover, OMMIPs could improve the intra-particle adsorption and thus provide a significant improvement in recognition sites availability over TBMIPs. Using the quercetin-OMMIPs as SPE sorbent, quercetin was directly extracted from the crude Gingko leaves extract with a satisfying selectivity and elution recovery.
在这项工作中，为了提高分子印迹聚合物（MIPs）的识别位点的可用性，通过将槲皮素-MIPs 层接枝到有序大孔巯基功能化硅的孔壁上，简便地制备了有序大孔分子印迹聚合物（OMMIPs）。通过 FTIR、Raman、SEM、BET 和 TGA 测量对孔结构进行了表征。结果表明，与传统的整体 MIPs（TBMIPs）相比，OMMIPs 具有纳米级聚合物层、更规则的大孔结构和更大的孔隙率。OMMIPs 的聚合物含量约为 49.7%。动力学和等温吸附实验表明，与结构类似物相比，OMMIPs 对槲皮素有更高的亲和力和选择性。此外，OMMIPs 可以改善颗粒内吸附，从而大大提高识别位点的可用性，优于 TBMIPs。使用槲皮素-OMMIPs 作为 SPE 吸附剂，可直接从银杏叶粗提取物中提取槲皮素，具有令人满意的选择性和洗脱回收率。
