University of California-Davis, 4625 2nd Ave, Suite 3005, Sacramento, CA 95817, USA.
University of California-Davis, 4625 2nd Ave, Suite 3005, Sacramento, CA 95817, USA; Shriners Hospitals for Children Northern California, 2425 Stockton Blvd, Sacramento, CA 95817, USA.
J Pediatr Surg. 2020 Sep;55(9):1941-1946. doi: 10.1016/j.jpedsurg.2019.09.029. Epub 2019 Oct 21.
We determined whether in vitro potency assays inform which placental mesenchymal stromal cell (PMSC) lines produce high rates of ambulation following in utero treatment of myelomeningocele in an ovine model.
PMSC lines were created following explant culture of three early-gestation human placentas. In vitro neuroprotection was assessed with a neuronal apoptosis model. In vivo, myelomeningocele defects were created in 28 fetuses and repaired with PMSCs at 3 × 10 cells/cm of scaffold from Line A (n = 6), Line B (n = 7) and Line C (n = 5) and compared to no PMSCs (n = 10). Ambulation was scored as ≥13 on the Sheep Locomotor Rating Scale.
In vitro, Line A and B had higher neuroprotective capability than no PMSCs (1.7 and 1.8 respectively vs 1, p = 0.02, ANOVA). In vivo, Line A and B had higher large neuron densities than no PMSCs (25.2 and 27.9 respectively vs 4.8, p = 0.03, ANOVA). Line C did not have higher neuroprotection or larger neuron density than no PMSCs. In vivo, Line A and B had ambulation rates of 83% and 71%, respectively, compared to 60% with Line C and 20% with no PMSCs.
The in vitro neuroprotection assay will facilitate selection of optimal PMSC lines for clinical use.
n/a.
Basic science.
我们旨在确定体外效力测定是否能预测哪种胎盘间充质基质细胞（PMSC）系在羊膜内治疗脊髓脊膜膨出的模型中能提高运动能力。
通过对三个早期妊娠胎盘进行外植体培养来创建 PMSC 系。用神经元凋亡模型评估体外神经保护作用。在体内，在 28 个胎儿中创建脊髓脊膜膨出缺陷，并使用来自 Line A（n=6）、Line B（n=7）和 Line C（n=5）的 PMSC 修复，细胞密度为 3×10 细胞/cm 支架，与不使用 PMSC 修复（n=10）进行比较。将运动能力评分≥13 分作为羊运动评分量表的评分标准。
在体外，Line A 和 Line B 的神经保护能力均高于无 PMSC（分别为 1.7 和 1.8 比 1，p=0.02，ANOVA）。在体内，Line A 和 Line B 的大神经元密度均高于无 PMSC（分别为 25.2 和 27.9 比 4.8，p=0.03，ANOVA）。Line C 的神经保护或更大神经元密度均不如无 PMSC。在体内，Line A 和 Line B 的运动能力分别为 83%和 71%，而 Line C 为 60%，无 PMSC 为 20%。
体外神经保护测定将有助于选择用于临床的最佳 PMSC 系。
无。
基础科学。
