Asadian Mahla, Azimi Leila, Alinejad Faranak, Ostadi Yalda, Lari Abdolaziz Rastegar
Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran Universitu of Medical Sciences, Tehran, Iran.
Pediatric Infections Research Center, Research Institute for Children's Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Adv Biomed Res. 2019 Sep 23;8:58. doi: 10.4103/abr.abr_256_18. eCollection 2019.
Multidrug-resistant can cause complications in antibiotic therapy and increase the rate of morbidity and mortality in hospitalized patients. Patients with ventilator and burns are two specific groups at high risk for infections. This study aimed to determine antibiotic susceptibility patterns associated with biofilm production in and to assess its molecular epidemiology by random amplified polymorphic DNA polymerase chain reaction (RAPD PCR) in isolated from ventilator-associated pneumonia and burn wound colonization.
In this study, 79 isolates of (32 ventilator-associated pneumonia [VAP] 47 burns) were collected in two teaching hospitals in Tehran, Iran, in 2018. Conventional biochemical and microbiological methods were used to identify bacteria. Antibiotic susceptibility was detected by disc diffusion methods according to the Clinical and Laboratory Standards Institute 2018. Tube test was examined for the detection of the biofilm formation rate in collected strains. The most prevalent carbapenemase genes were detected by PCR and molecular typing by RAPD PCR.
All of bacteria were extensively drug-resistant (XDR) except for two isolates. The results of tube test indicated that only 36% of XDR strains were in weak rate of biofilm formation group. Two major clonal genetic groups were found in VAP and burn strains. Oxa-23 was the most prevalent carbapenemase in collected .
The presence of XDR strains of is considerable significant problem in hospitals. Further, similar genetic clonal identified in them indicated the nosocomial infection origin. Hence, these results are very important for control of nosocomial infection committee in health-care systems.
多重耐药菌可导致抗生素治疗出现并发症，并增加住院患者的发病率和死亡率。使用呼吸机的患者和烧伤患者是两类感染风险较高的特定人群。本研究旨在确定与生物膜产生相关的抗生素敏感性模式，并通过随机扩增多态性DNA聚合酶链反应（RAPD-PCR）评估从呼吸机相关性肺炎和烧伤创面定植中分离出的多重耐药菌的分子流行病学。
在本研究中，2018年在伊朗德黑兰的两家教学医院收集了79株多重耐药菌（32株来自呼吸机相关性肺炎[VAP]，47株来自烧伤）。采用传统的生化和微生物学方法鉴定细菌。根据2018年临床和实验室标准协会的标准，通过纸片扩散法检测抗生素敏感性。通过试管法检测所收集菌株的生物膜形成率。通过PCR检测最常见的碳青霉烯酶基因，并通过RAPD-PCR进行分子分型。
除两株分离菌外，所有细菌均为广泛耐药（XDR）菌。试管法结果表明，只有36%的XDR菌株处于生物膜形成率较弱的组。在VAP和烧伤菌株中发现了两个主要的克隆基因组。Oxa-23是所收集的多重耐药菌中最常见的碳青霉烯酶。
医院中存在广泛耐药的多重耐药菌菌株是一个相当严重的问题。此外，在它们中鉴定出的相似遗传克隆表明了医院感染的来源。因此，这些结果对于医疗保健系统中控制医院感染委员会非常重要。
