Respiratory and Meningeal Diseases Section, Central Public Health Laboratory, Asunción, Paraguay.
Pan American Health Organization / World Health Organization, Washington, DC, USA.
J Med Microbiol. 2019 Dec;68(12):1793-1801. doi: 10.1099/jmm.0.001097.
Colonization by is the pre-requisite for the development of disease. We present the findings of a cross-sectional investigation onto the oropharyngeal carriage of and species in the population aged 3 to 21 in Paraguay. Carriage prevalence by age groups, risk factors associated with carriage, and phenotypic and genotypic characteristics of strains are described. We collected 2011 oropharyngeal swabs from consenting participants aged 3-21 years. Infants were recruited at immunization clinics, and older children and young adults were identified at schools and universities. A single oropharyngeal swab was collected and processed for the identification and isolation of . Additionally, participants, or their legal guardian if these were minors, were requested to fill a standardized questionnaire. was isolated in 42/2011 (2.1 %) participants, while other spp. were identified in 306/2011 (15.2 %) subjects: and were identified in 39/2011 (1.9 %) and 43/2011 (2.2 %), respectively. Meningococcal strains belonged to ten different clonal complexes, of which six are associated with invasive disease (ST-32/ET5 complex, ST-11/ET37 complex, ST-103 complex, ST-167 complex, ST-35 complex and ST-41/44 complex/lineage 3). Prevalence of carriage was low compared to that reported from other settings, however, the overall carriage of spp. (including ) was comparable to meningococcal carriage prevalence reported in the literature. This study is the first of its kind conducted in Paraguay, and one of the few known in the Southern Cone of Latin America.
定植是疾病发生的前提。我们报告了一项横断面研究的结果，该研究调查了巴拉圭 3 至 21 岁人群的口咽部携带和 种的情况。描述了按年龄组的携带率、与携带相关的危险因素，以及菌株的表型和基因型特征。我们收集了 2011 名 3-21 岁同意参与者的咽拭子。婴儿在免疫接种诊所招募，年龄较大的儿童和年轻人在学校和大学招募。采集单个咽拭子进行鉴定和分离 。此外，要求参与者（如果是未成年人，则为其法定监护人）填写一份标准化问卷。42/2011（2.1%）的参与者中分离出 ，而 306/2011（15.2%）的其他 spp.：39/2011（1.9%）和 43/2011（2.2%）分别鉴定出 和 。脑膜炎球菌菌株属于十个不同的克隆复合体，其中六个与侵袭性疾病有关（ST-32/ET5 复合体、ST-11/ET37 复合体、ST-103 复合体、ST-167 复合体、ST-35 复合体和 ST-41/44 复合体/谱系 3）。与其他地区报告的情况相比， 携带率较低，但总的 spp.（包括 ）携带率与文献中报道的脑膜炎球菌携带率相当。这是巴拉圭进行的首例此类研究，也是拉丁美洲南部锥体地区为数不多的已知研究之一。
