Qin Xing, Liu Jinlin, Zhang Qing, Chen Wantao, Zhong Xiaoxia, He Jie
Department of Oral and Maxillofacial-Head and Neck Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai, 200011, China.
Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, Shanghai, 200011, People's Republic of China.
Nanoscale Res Lett. 2021 Jan 21;16(1):14. doi: 10.1186/s11671-021-03478-2.
In recent years, multifunctional nanoparticles with combined diagnostic and therapeutic functions show great promise in nanomedicine. In this study, we report the environmentally friendly synthesis of fluorescent carbon nano-dots such as carbon quantum dots (CQDs) by microplasma using o-phenylenediamine. The produced CQDs exhibited a wide absorption peaks at 380-500 nm and emitted bright yellow fluorescence with a peak at 550 nm. The CQDs were rapidly taken up by HeLa cancer cells. When excited under blue light, a bright yellow fluorescence signal and intense reactive oxygen species (ROS) were efficiently produced, enabling simultaneous fluorescent cancer cell imaging and photodynamic inactivation, with a 40% decrease in relative cell viability. Furthermore, about 98% cells were active after the incubation with 400 μg mL CQDs in the dark, which revealed the excellent biocompatibility of CQDs. Hence, the newly prepared CQDs are thus demonstrated to be materials which might be effective and safe to use for in vivo bioimaging and imaging-guided cancer therapy.
近年来，具有诊断和治疗双重功能的多功能纳米粒子在纳米医学领域展现出了巨大的潜力。在本研究中，我们报道了利用邻苯二胺通过微等离子体环境友好地合成荧光碳纳米点，如碳量子点（CQDs）。所制备的碳量子点在380 - 500 nm处呈现出较宽的吸收峰，并发射出峰值位于550 nm的亮黄色荧光。碳量子点能被HeLa癌细胞快速摄取。在蓝光激发下，能高效产生亮黄色荧光信号和大量活性氧（ROS），实现了对癌细胞的同步荧光成像和光动力灭活，相对细胞活力降低了40%。此外，在黑暗中用400 μg/mL碳量子点孵育后，约98%的细胞仍具有活性，这表明碳量子点具有优异的生物相容性。因此，新制备的碳量子点被证明是可有效且安全地用于体内生物成像和成像引导癌症治疗的材料。
