MOE Medical Devices LLC, Valhalla, NY, USA.
Department of Microbiology and Immunology, New York Medical College, Valhalla, NY, USA.
Mycoses. 2020 Feb;63(2):225-232. doi: 10.1111/myc.13030. Epub 2019 Dec 15.
Onychomycosis affects almost 6% of the world population. Topical azoles and systemic antifungal agents are of low efficacy and can have undesirable side effects. An effective, non-invasive therapy for onychomycosis is an unmet clinical need.
Determine the efficacy threshold of non-thermal atmospheric plasma (NTAP) to treat onychomycosis in an in vitro model.
A novel toe/nail-plate model using cadaver nails and agarose media inoculated with Candida albicans was exposed to a range of NTAP doses.
Direct exposure of C albicans and Trichophyton mentagrophytes to 12 minutes of NTAP results in complete killing at doses of 39 and 15 kPulses, respectively. Onset of reduced viability of C albicans to NTAP treatment through the nail plate occurs at 64 kPulses with 10× and 100× reduction at 212 and 550 kPulses, respectively.
NTAP is an effective, non-invasive therapeutic approach to onychomycosis that should be evaluated in a clinical setting.
甲真菌病影响全球近 6%的人口。局部唑类药物和系统抗真菌药物疗效低，且可能有不良副作用。甲真菌病的有效、非侵入性治疗方法是临床未满足的需求。
在体外模型中确定非热大气压等离子体（NTAP）治疗甲真菌病的疗效阈值。
使用尸体指甲和接种白色念珠菌的琼脂培养基的新型脚趾/指甲板模型，暴露于一系列 NTAP 剂量下。
白色念珠菌和须癣毛癣菌直接暴露于 12 分钟的 NTAP 中，分别在 39 和 15 kPulses 的剂量下完全杀灭。通过指甲板对 NTAP 治疗的白色念珠菌活力降低的起始剂量为 64 kPulses，分别在 212 和 550 kPulses 时降低 10×和 100×。
NTAP 是一种有效、非侵入性的治疗甲真菌病的方法，应在临床环境中进行评估。
