非热常压等离子体处理体外人指甲模型中的甲真菌病。
Non-thermal atmospheric plasma treatment of onychomycosis in an in vitro human nail model.
机构信息
MOE Medical Devices LLC, Valhalla, NY, USA.
Department of Microbiology and Immunology, New York Medical College, Valhalla, NY, USA.
出版信息
Mycoses. 2020 Feb;63(2):225-232. doi: 10.1111/myc.13030. Epub 2019 Dec 15.
BACKGROUND
Onychomycosis affects almost 6% of the world population. Topical azoles and systemic antifungal agents are of low efficacy and can have undesirable side effects. An effective, non-invasive therapy for onychomycosis is an unmet clinical need.
OBJECTIVE
Determine the efficacy threshold of non-thermal atmospheric plasma (NTAP) to treat onychomycosis in an in vitro model.
METHODS
A novel toe/nail-plate model using cadaver nails and agarose media inoculated with Candida albicans was exposed to a range of NTAP doses.
RESULTS
Direct exposure of C albicans and Trichophyton mentagrophytes to 12 minutes of NTAP results in complete killing at doses of 39 and 15 kPulses, respectively. Onset of reduced viability of C albicans to NTAP treatment through the nail plate occurs at 64 kPulses with 10× and 100× reduction at 212 and 550 kPulses, respectively.
CONCLUSIONS
NTAP is an effective, non-invasive therapeutic approach to onychomycosis that should be evaluated in a clinical setting.
背景
甲真菌病影响全球近 6%的人口。局部唑类药物和系统抗真菌药物疗效低，且可能有不良副作用。甲真菌病的有效、非侵入性治疗方法是临床未满足的需求。
目的
在体外模型中确定非热大气压等离子体（NTAP）治疗甲真菌病的疗效阈值。
方法
使用尸体指甲和接种白色念珠菌的琼脂培养基的新型脚趾/指甲板模型，暴露于一系列 NTAP 剂量下。
结果
白色念珠菌和须癣毛癣菌直接暴露于 12 分钟的 NTAP 中，分别在 39 和 15 kPulses 的剂量下完全杀灭。通过指甲板对 NTAP 治疗的白色念珠菌活力降低的起始剂量为 64 kPulses，分别在 212 和 550 kPulses 时降低 10×和 100×。
结论
NTAP 是一种有效、非侵入性的治疗甲真菌病的方法，应在临床环境中进行评估。
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