Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk, 80-416 Gdańsk, Poland.
Acta Pharm. 2020 Mar 1;70(1):89-99. doi: 10.2478/acph-2020-0013.
Fenugreek seeds are known as a source of various compounds, the most common of which are steroidal saponins. However, despite the growing interest in this plant material as a healing agent, spice and dietary supplement ingredient, the composition of Polish fenugreek seeds remains unknown. Therefore, the steroidal saponin complex in the seeds of T. foenum-graecum cultivated in Poland was qualitatively and quantitatively analyzed by the HPLC-ELSDESI-MS method. Two C-18 columns connected in series were used for the first time in analysis of fenugreek saponins and ELS detector parameters were optimized. A total of 26 furostanol saponins were revealed, of which 24 were tentatively identified. The HPLC-ELSD method developed for quantitative analysis was preliminarily validated and the determined amount of steroidal saponins in Polish fenugreek seeds was 0.14 %.
葫芦巴种子被认为是各种化合物的来源，其中最常见的是甾体皂苷。然而，尽管人们对这种植物材料作为治疗剂、香料和膳食补充剂成分的兴趣日益浓厚，但波兰葫芦巴种子的成分仍不清楚。因此，采用 HPLC-ELSD-ESI-MS 方法对波兰种植的 T. foenum-graecum 种子中的甾体皂苷复合物进行了定性和定量分析。首次在分析葫芦巴皂苷时使用了串联连接的两个 C-18 柱，并对 ELS 检测器参数进行了优化。总共揭示了 26 种呋甾醇皂苷，其中 24 种被初步鉴定。为定量分析开发的 HPLC-ELSD 方法进行了初步验证，测定的波兰葫芦巴种子中甾体皂苷的含量为 0.14%。
