Institute of Tropical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
Sci-tech Industrial Park, Guangzhou University of Chinese Medicine, Guangzhou 510445, China.
Molecules. 2018 Dec 10;23(12):3256. doi: 10.3390/molecules23123256.
As a folk medicine, L. is used effectively to treat inflammatory conditions and skin diseases. However, its mechanism of action is not well understood, limiting its medical use. We isolated and identified three compounds, namely niazirin, marumoside A and sitosterol----d-glucoside, from the seeds of , and studied their effects on the expression of Th17-relevant cytokines (IL-12/IL-23 p40, IL-17A, IL-22 and IL-23 p19) using lipopolysaccharide-stimulated THP-1 cells. Additionally, as Th17 plays a critical role in the pathogenesis of psoriasis, we used a --tetradecanoylphorbol--acetate (TPA)-induced psoriasis-like skin lesion mouse model to study their potential therapeutic application in vivo. The compounds suppressed the expression of IL-12/IL-23 p40, IL-17A, IL-22 and IL-23 p19 in vitro, and in vivo they ameliorated psoriasis-like skin lesions, decreased IL-17A mRNA expression, and increased the expression of keratinocyte differentiation markers. To our knowledge, this is the first report regarding the mechanism and therapeutic application of seeds to treat psoriasis-like lesions in vivo.
作为一种民间药物，[药物名称]被有效地用于治疗炎症性疾病和皮肤病。然而，其作用机制尚不清楚，限制了它在医学上的应用。我们从[药物名称]的种子中分离并鉴定了三种化合物，即 niazirin、marumoside A 和 sitosterol----d-glucoside，并研究了它们对脂多糖刺激的 THP-1 细胞中 Th17 相关细胞因子（IL-12/IL-23 p40、IL-17A、IL-22 和 IL-23 p19）表达的影响。此外，由于 Th17 在银屑病发病机制中起着关键作用，我们使用十四烷酰佛波醇乙酸酯（TPA）诱导的银屑病样皮肤损伤小鼠模型研究了它们在体内的潜在治疗应用。这些化合物在体外抑制了 IL-12/IL-23 p40、IL-17A、IL-22 和 IL-23 p19 的表达，在体内改善了银屑病样皮肤损伤，降低了 IL-17A mRNA 的表达，并增加了角质形成细胞分化标志物的表达。据我们所知，这是首次报道[药物名称]种子在体内治疗银屑病样病变的机制和治疗应用。
