Oral Medicine, Oral Surgery and Implantology Unit, Faculty of Medicine and Odontology, University of Santiago de Compostela, Foundation of Health Research Institute of Santiago de Compostela, Santiago de Compostela, Spain.
Department of Pathology, Clinical University Hospital, Santiago de Compostela, Spain.
Cancer Biomark. 2020;27(1):95-103. doi: 10.3233/CBM-190776.
Oral squamous cell carcinoma (OSCC) represents 95% of all cancers of the head and neck region. The five-year survival rate of OSCC patients is about 60% and has not gone throw significant improvements despite recent advances in molecular biology, or the identification of key pathways in its pathophysiology such as cell cycle.
Immunohistochemistry (IHC) for p16INK4a, p21CIP1, cyclin D1 and p27KIP1 protein expression was quantified by tissue microarray analysis in 68 samples from OSCC patients. In order to analyse its correlation with genetic information, a cohort belonging to The Cancer Genome Atlas (TCGA) database was analysed.
Of 68 patients, 34 (50%) developed recurrence, and 36 (52.09%) died as a result of disease progression (mean survival 34.09 months). IHC staining for nuclear cyclin D1 was associated with worse staging and tobacco use. p16INK4a, p21CIP1, cyclin D1, and p27KIP1 expression was unrelated to overall survival. No statistically significant correlation linked the CPs-related genes mutations to OSCC overall survival in the TCGA database.
CPs variations at a phenotype and genotype level seem not to affect significantly clinicopathological features and survival in the studied OSCC cohorts.
口腔鳞状细胞癌（OSCC）占头颈部区域所有癌症的 95%。尽管近年来在分子生物学方面取得了进展，或在其病理生理学的关键途径（如细胞周期）方面确定了关键途径，但 OSCC 患者的五年生存率约为 60%，并未有显著改善。
1）分析 OSCC 机构队列中细胞周期检查点（CPs）的免疫表达，并将其与临床病理特征和生存相关联，2）研究 TCGA 数据库中 OSCC 亚组的 CPs 相关基因。
通过组织微阵列分析对 68 例 OSCC 患者的 p16INK4a、p21CIP1、cyclin D1 和 p27KIP1 蛋白表达进行免疫组化（IHC）定量。为了分析其与遗传信息的相关性，分析了属于癌症基因组图谱（TCGA）数据库的队列。
在 68 例患者中，34 例（50%）发生复发，36 例（52.09%）因疾病进展而死亡（平均生存时间为 34.09 个月）。核 cyclin D1 的 IHC 染色与较差的分期和吸烟有关。p16INK4a、p21CIP1、cyclin D1 和 p27KIP1 表达与总生存无关。在 TCGA 数据库中，CPs 相关基因的突变与 OSCC 总生存率之间没有统计学上显著的相关性。
在研究的 OSCC 队列中，CPs 在表型和基因型水平上的变化似乎不会显著影响临床病理特征和生存。
