Sekla L, Stackiw W, Eibisch G, Johnson I
Cadham Provincial Laboratory, Winnipeg, Manitoba.
Clin Invest Med. 1988 Aug;11(4):304-9.
During an epidemic of measles in a vaccinated community, five serodiagnostic tests were performed on 67 persons on whom clinical and epidemiological data were available. The test found most suitable for a rapid diagnosis of measles infection was an Enzyme Linked Immuno Sorbent Assay for the detection of specific IgM antibodies. Only one false negative IgM was recorded. In a group of 45 persons who fulfilled the clinical definition of measles, specific IgM antibodies were detected in the acute phase serum of only 30 (66.6%), of whom 17 were vaccinated. When the convalescent sera were tested, specific IgM antibodies were detected in 25 of the 28 (89.2%) vaccinated, and in 17 of the 17 (100%) non vaccinated clinical cases. A convalescent blood should be tested in persons with a rash illness and no IgM antibodies in the acute phase serum. There were individual variations in the time of appearance of IgM. On the day of onset of rash, IgM antibodies were detected in 7 of the 12 (58.3%). A history of prior vaccination is not always associated with immunity nor with the presence of specific antibodies.
在一个已接种疫苗的社区发生麻疹疫情期间，对67名有临床和流行病学数据的人员进行了5种血清学诊断检测。最适合快速诊断麻疹感染的检测方法是用于检测特异性IgM抗体的酶联免疫吸附测定法。仅记录到1例假阴性IgM。在一组符合麻疹临床定义的45人中，仅在30人（66.6%）的急性期血清中检测到特异性IgM抗体，其中17人接种过疫苗。检测恢复期血清时，在28名接种过疫苗的人中，有25人（89.2%）检测到特异性IgM抗体，在17名未接种疫苗的临床病例中，17人（100%）检测到特异性IgM抗体。对于出疹性疾病且急性期血清中无IgM抗体的人员，应检测恢复期血液。IgM出现的时间存在个体差异。出疹当天，12人中有7人（58.3%）检测到IgM抗体。既往接种疫苗史并不总是与免疫或特异性抗体的存在相关。
