用于鉴定猪骨髓来源树突状细胞的PD9-9单克隆抗体的研制

Development of the PD9-9 Monoclonal Antibody for Identifying Porcine Bone Marrow-Derived Dendritic Cells.

作者信息

Kim Sang Eun, Kim Young Kyu, Oh Keon Bong, Hwang Jeong Ho

机构信息

Department of Stem Cell and Regenerative Biotechnology, KU Institute of Science and Technology (KIT), Konkuk University, Seoul 05029, Republic of Korea.

Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju-gun 55365, Republic of Korea.

出版信息

Life (Basel). 2024 Aug 23;14(9):1054. doi: 10.3390/life14091054.

DOI:10.3390/life14091054

PMID:39337839

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11433566/

Abstract

The purpose of this study was to develop a monoclonal antibody (mAb) that can identify porcine dendritic cells (DCs) that have differentiated from bone marrow progenitor cells. Hybridoma technology was used to obtain mAbs, and bone marrow-derived DCs (BMDCs) were employed as immunogens for producing antibodies. The generated PD9-9 mAbs exhibited considerable reactivity towards porcine BMDCs with applications in flow cytometry and immunostaining. The antibody was composed of heavy immunoglobulin gamma-1 chains and light kappa chains. The PD9-9 mAb recognized fully differentiated porcine BMDCs and cells undergoing DC differentiation. In contrast, bone marrow cells and macrophages were not recognized by PD9-9. In addition, the PD9-9 mAb promoted porcine DC proliferation. Consequently, the PD9-9 mAb may be a biomarker for porcine DCs and will be advantageous for investigating porcine DC biology.

摘要

本研究的目的是开发一种单克隆抗体（mAb），该抗体能够识别已从骨髓祖细胞分化而来的猪树突状细胞（DCs）。采用杂交瘤技术获得单克隆抗体，并将骨髓来源的DCs（BMDCs）用作产生抗体的免疫原。所产生的PD9-9单克隆抗体对猪BMDCs表现出显著的反应性，可应用于流式细胞术和免疫染色。该抗体由重链免疫球蛋白γ-1链和轻链κ链组成。PD9-9单克隆抗体识别完全分化的猪BMDCs以及正在进行DC分化的细胞。相比之下，骨髓细胞和巨噬细胞不能被PD9-9识别。此外，PD9-9单克隆抗体促进猪DC增殖。因此，PD9-9单克隆抗体可能是猪DCs的一种生物标志物，将有利于研究猪DC生物学。