Balikesir University, Faculty of Medicine, Department of Pathology, Balikesir, Turkey.
Balikesir University, Faculty of Medicine Department of Otolaryngology, Balikesir, Turkey.
Anal Cell Pathol (Amst). 2019 Oct 8;2019:3563215. doi: 10.1155/2019/3563215. eCollection 2019.
To evaluate the effect of streptozotocin-induced experimental diabetes mellitus on p16, p53, Ki67, and Bcl2 expressions and histopathological changes in the tongue of the rats.
Twenty-two adult female Sprague-Dawley rats were used. The rats were randomly divided into 2 groups ( = 14) as control (C) ( = 8) and diabetic (DM) ( = 6). The rats in the DM group were given streptozotocin as a single intraperitoneal dose for induction of diabetes. Histopathological and immunohistochemical evaluations of formalin-fixed and paraffin-embedded tissue sections of the tongue were used.
Significant differences were observed between the DM group and the control group in terms of epithelial thickness, length of filiform papillae, and width of filiform papillae ( = 0.005, = 0.001, and = 0.006, respectively). There was no significant difference between the groups in terms of mononuclear inflammatory cell infiltration, capillary proliferation, and dysplasia ( = 0.204, = 0.244, and = 0.204, respectively). As a result of immunohistochemical studies, no significant difference was found between the groups in terms of p53, Ki67, and Bcl-2 expressions ( = 0.588, = 0.662, and = 0.686, respectively). A significant difference was found between the groups when p16 expression was evaluated ( = 0.006).
In our study, streptozotocin-induced experimental diabetes mellitus induced p16 expression but did not show any difference in p53, Bcl-2, and Ki67 levels. It should be considered in the studies that the pathological changes at the early stages of the relationship between DM and oral cancer may be related to p16 expression; however, it may also be linked with p16-related aging process.
评估链脲佐菌素诱导的实验性糖尿病对大鼠舌组织中 p16、p53、Ki67 和 Bcl2 表达及组织病理学变化的影响。
使用 22 只成年雌性 Sprague-Dawley 大鼠。将大鼠随机分为 2 组（n = 14）：对照组（C）（n = 8）和糖尿病组（DM）（n = 6）。DM 组大鼠单次腹腔内注射链脲佐菌素诱导糖尿病。使用福尔马林固定和石蜡包埋组织切片进行组织病理学和免疫组织化学评价。
DM 组与对照组在舌上皮厚度、丝状乳头长度和宽度方面存在显著差异（P 值分别为 0.005、0.001 和 0.006）。两组在单核炎性细胞浸润、毛细血管增生和发育不良方面无显著差异（P 值分别为 0.204、0.244 和 0.204）。免疫组织化学研究结果显示，两组在 p53、Ki67 和 Bcl-2 表达方面无显著差异（P 值分别为 0.588、0.662 和 0.686）。当评估 p16 表达时，两组之间存在显著差异（P = 0.006）。
在本研究中，链脲佐菌素诱导的实验性糖尿病诱导了 p16 的表达，但 p53、Bcl-2 和 Ki67 水平没有差异。在 DM 与口腔癌关系的早期阶段，病理变化可能与 p16 表达有关；然而，这也可能与 p16 相关的衰老过程有关。
