Doris P A
Department of Cell Biology and Anatomy, Texas Tech University School of Medicine, Lubbock 79430.
Endocrinology. 1988 Nov;123(5):2440-4. doi: 10.1210/endo-123-5-2440.
A study has been performed to investigate the tissue of origin of an endogenous plasma factor which has been previously shown to bind to an antidigoxin antibody raised in this laboratory. Adult male rats were used to provide tissue, which was homogenized ultrasonically and extracted on C18 minicolumns. The extracts were then assayed in a RIA for digoxin, and the ability of tissue extracts to displace radioiodinated digoxin from its antibody was determined. Consistent displacement was produced by extracts of whole adrenal gland, but not by whole brain and brain regions, anterior or posterior pituitary gland, skeletal muscle, aorta, testis, kidney, spleen, atrium, or ventricle. In some samples, extracts of liver also produced displacement of digoxin tracer. Further experiments were performed to determine whether bilateral adrenalectomy was able to influence plasma levels of the endogenous digitalis-like factor. Forty-eight hours after adrenalectomy, plasma levels of the factor were significantly reduced in whole plasma and ether extracts of plasma and were insignificantly lower in C18 extracts of plasma. These findings suggest that the endogenous factor we have studied previously in plasma may be derived from the adrenal gland.
一项研究旨在调查一种内源性血浆因子的组织来源，该因子先前已被证明能与本实验室制备的抗地高辛抗体结合。使用成年雄性大鼠提供组织，将其超声匀浆并在C18微柱上进行提取。然后将提取物用于地高辛的放射免疫分析，并测定组织提取物从其抗体中置换放射性碘标记地高辛的能力。全肾上腺提取物能产生一致的置换作用，但全脑及脑区、垂体前叶或后叶、骨骼肌、主动脉、睾丸、肾脏、脾脏、心房或心室的提取物则不能。在一些样本中，肝脏提取物也能使地高辛示踪剂发生置换。进一步的实验旨在确定双侧肾上腺切除术是否能够影响内源性洋地黄样因子的血浆水平。肾上腺切除术后48小时，全血浆及血浆乙醚提取物中该因子的血浆水平显著降低，而血浆C18提取物中的降低则不显著。这些发现表明，我们先前在血浆中研究的内源性因子可能来源于肾上腺。
