Chen Xi, Wang Wenjing, Li Ruien, Yu Jing, Gao Lei
Endocrine Metabolic Disease Section.
Anorectal Department, the Affiliated Hospital to Changchun University of Chinese Medicine.
Medicine (Baltimore). 2019 Nov;98(44):e17519. doi: 10.1097/MD.0000000000017519.
Accumulated evidence has indicated the associations between single-nucleotide polymorphisms (SNPs) in microRNAs (miRNAs) and the susceptibility to diabetes mellitus (DM), but the conclusions remain controversial. This study was to investigate the true contribution of miRNA SNPs to the risk of DM by using a meta-analysis of all the published studies.
Relevant studies were identified in the databases of PubMed and the Cochrane Library databases. The strength of associations between miRNA polymorphisms and DM risk was assessed by odds ratios (ORs) and 95% confidence intervals (95% CIs) under five genetic models using the STATA software.
Six studies, containing 2773 cases and 2632 controls, were enrolled, 5 of which evaluated miR-146a (rs2910164), 4 for miR-27a (rs895819), and 3 for miR-124 (rs531564) and 2 for miR-375 (rs6715345), miR-128a (rs11888095), miR-194a (rs3820455). The meta-analysis indicated that the G allele or GG genotype of miR-146a rs2910164 was associated with a significantly increased risk for DM compared with C allele or GC/CC genotype in Latin American population; CC genotype of miR-27a rs895819 polymorphism was associated with a significantly decreased risk for DM in Asian population compared with the TT genotype; patients carrying with CC genotype of miR-124 rs531564 had a lower probability to develop DM regardless of ethnicity; no associations were identified between polymorphisms in miR-375, miR-128a, miR-194a and the susceptibility to DM.
Our study suggests that miR-146a/miR-27a and miR-124 polymorphisms may be ethnicity-dependent or -independent susceptibility factors to DM, respectively.
越来越多的证据表明，微小RNA（miRNA）中的单核苷酸多态性（SNP）与糖尿病（DM）易感性之间存在关联，但结论仍存在争议。本研究旨在通过对所有已发表研究进行荟萃分析，探讨miRNA SNP对DM风险的真正影响。
在PubMed数据库和Cochrane图书馆数据库中检索相关研究。使用STATA软件，在五种遗传模型下，通过比值比（OR）和95%置信区间（95%CI）评估miRNA多态性与DM风险之间的关联强度。
纳入6项研究，共2773例病例和2632例对照，其中5项评估miR-146a（rs2910164），4项评估miR-27a（rs895819），3项评估miR-124（rs531564），2项评估miR-375（rs6715345）、miR-128a（rs11888095）、miR-194a（rs3820455）。荟萃分析表明，在拉丁裔人群中，与C等位基因或GC/CC基因型相比，miR-146a rs2910164的G等位基因或GG基因型与DM风险显著增加相关；在亚洲人群中，与TT基因型相比，miR-27a rs895819多态性的CC基因型与DM风险显著降低相关；无论种族如何，携带miR-124 rs531564的CC基因型的患者患DM的概率较低；未发现miR-375、miR-128a、miR-194a的多态性与DM易感性之间存在关联。
我们的研究表明，miR-146a/miR-27a和miR-124多态性可能分别是DM的种族依赖性或非依赖性易感因素。
