Kuo Shu-Jui, Hsua Ping-Wen, Chien Szu-Yu, Huang Chien-Chung, Hu Sung-Lin, Tsai Chun-Hao, Su Chen-Ming, Tang Chih-Hsin
School of Medicine.
Department of Orthopedic Surgery.
Medicine (Baltimore). 2019 Nov;98(44):e17604. doi: 10.1097/MD.0000000000017604.
This study genotyped blood samples from 214 patients with rheumatoid arthritis (RA) and 293 healthy controls for single nucleotide polymorphisms (SNPs) rs2977537, rs2929970, rs2929973, rs2977530, rs1689334 and rs62514004. We want to investigate whether the SNPs in the WNT1-inducible signaling pathway protein 1 (WISP-1) gene may increase the risk of developing RA. We showed that RA disease was more likely with the AA genotype compared with the AG genotype of SNP rs2977537 (adjusted odds ratio [AOR]: 0.54; 95% confidence interval [CI]: 0.34-0.84), and with the TT genotype (AOR: 0.24; 95% CI: 0.13-0.39) or the GG genotype (AOR: 0.05; 95% CI: 0.03-0.10) compared with the GT genotype of rs2929973, and with the AA genotype (AOR: 0.34; 95% CI: 0.22-0.54) or GG genotype (AOR: 0.52; 95% CI: 0.31 to 0.87) vs the AG genotype of rs2977530. Rheumatoid factor positivity was more likely with the AA genotype than with the AG genotype of the rs2977537 polymorphism (AOR: 0.16; 95% CI: 0.16-0.94). High CRP (>8 mg/L) was more likely with the non-AG genotype (AA + GG) than the AG genotype of rs2977537 (AOR: 1.84; 95% CI: 1.05-3.21) and with the AA genotype vs the AG genotype of rs2977530 (AOR: 2.62; 95% CI: 1.35-5.09). Compared with the AG genotype, the AA genotype of rs2929970 was more likely to require prednisolone (AOR: 0.49; 95% CI: 0.27-0.88), while the AG genotype was more likely than the AA genotype of SNP rs2977530 to require TNF-α inhibitors (AOR: 2.07; 95% CI: 1.08 to 3.98). WISP-1 may be a diagnostic marker and therapeutic target for RA therapy.
本研究对214例类风湿性关节炎（RA）患者和293名健康对照者的血液样本进行基因分型，检测单核苷酸多态性（SNP）rs2977537、rs2929970、rs2929973、rs2977530、rs1689334和rs62514004。我们想研究WNT1诱导信号通路蛋白1（WISP-1）基因中的单核苷酸多态性是否可能增加患类风湿性关节炎的风险。我们发现，与SNP rs2977537的AG基因型相比，AA基因型患RA疾病的可能性更大（校正比值比[AOR]：0.54；95%置信区间[CI]：0.34 - 0.84）；与rs2929973的GT基因型相比，TT基因型（AOR：0.24；95% CI：0.13 - 0.39）或GG基因型（AOR：0.05；95% CI：0.03 - 0.10）患RA疾病的可能性更大；与rs2977530的AG基因型相比，AA基因型（AOR：0.34；95% CI：0.22 - 0.54）或GG基因型（AOR：0.52；95% CI：0.31至0.87）患RA疾病的可能性更大。rs2977537多态性的AA基因型比AG基因型类风湿因子阳性的可能性更大（AOR：0.16；95% CI：0.16 - 0.94）。与rs2977537的AG基因型相比，非AG基因型（AA + GG）出现高CRP（>8 mg/L）的可能性更大（AOR：1.84；95% CI：1.05 - 3.21），且与rs2977530的AA基因型相比，AG基因型出现高CRP的可能性更大（AOR：2.62；95% CI：1.35 - 5.09）。与AG基因型相比，rs2929970的AA基因型更有可能需要泼尼松龙治疗（AOR：0.49；95% CI：0.27 - 0.88），而与rs2977530的AA基因型相比，SNP rs2977530的AG基因型更有可能需要肿瘤坏死因子-α抑制剂治疗（AOR：2.07；95% CI：1.08至3.98）。WISP-1可能是RA治疗的诊断标志物和治疗靶点。
