利用微流道中的行波实现快速无添加剂的细菌裂解。

Rapid additive-free bacteria lysis using traveling surface acoustic waves in microfluidic channels.

机构信息

State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710049, People's Republic of China and School of Engineering and Applied Sciences, Harvard University, Cambridge, USA.

School of Engineering and Applied Sciences, Harvard University, Cambridge, USA.

出版信息

Lab Chip. 2019 Dec 21;19(24):4064-4070. doi: 10.1039/c9lc00656g. Epub 2019 Nov 6.

DOI:10.1039/c9lc00656g

PMID:31690904

Abstract

We report an additive-free method to lyse bacteria and extract nucleic acids and protein using a traveling surface acoustic wave (TSAW) coupled to a microfluidic device. We characterize the effects of the TSAW on E. coli by measuring the viability of cells exposed to the sound waves and find that about 90% are dead. In addition, we measure the protein and nucleic acids released from the cells and show that we recover about 20% of the total material. The lysis method should work for all types of bacteria. These results demonstrate the feasibility of using TSAW to lyse bacteria in a manner that is independent of the type of bacteria.

摘要

我们报告了一种无添加剂的方法，使用 traveling surface acoustic wave（TSAW）与微流控装置相结合来裂解细菌并提取核酸和蛋白质。我们通过测量暴露于声波的细胞的存活率来表征 TSAW 对大肠杆菌的影响，发现约 90%的细胞死亡。此外，我们还测量了从细胞中释放出的蛋白质和核酸，并表明我们回收了约 20%的总物质。该裂解方法应该适用于所有类型的细菌。这些结果表明，使用 TSAW 裂解细菌的方法在不依赖于细菌类型的情况下是可行的。