Department of Animal Health, Experimental Zooprophylactic Institute of Southern Italy, Via Salute, 2, 80055, Portici, NA, Italy.
Aquaculture Division, CAISIAL Center, University of Naples Federico II, Via Salute, Portici, NA, Italy.
Food Environ Virol. 2020 Mar;12(1):48-57. doi: 10.1007/s12560-019-09413-0. Epub 2019 Nov 6.
Bivalve mollusks as filter-feeders concentrate in their digestive tissue microorganisms likely present in the harvesting water, thus becoming risky food especially if consumed raw or poorly cooked. To eliminate bacteria and viruses eventually accumulated, they must undergo a depuration process which efficacy on viruses is on debate. To better clarify the worth of the depuration process on virus elimination from mussels, in this study we investigated rotavirus kinetics of accumulation and depuration in Mytilus galloprovincialis experimentally contaminated. Depuration process was monitored for 9 days and virus residual presence and infectivity were evaluated by real time quantitative polymerase chain reaction, cell culture and electron microscopy at days 1, 2, 3, 5, 7, 9 of depuration. Variables like presence of ozone and of microalgae feeding were also analyzed as possible depuration enhancers. Results showed a two-phase virus removal kinetic with a high decrease in the first 24 h of depuration and 5 days necessary to completely remove rotavirus.
双壳贝类作为滤食动物，会在其消化组织中浓缩可能存在于收获水中的微生物，因此成为高风险的食物，尤其是生吃或烹饪不当的情况下。为了消除最终积累的细菌和病毒，它们必须经过净化过程，但关于该过程对病毒的效果仍存在争议。为了更好地阐明净化过程在从贻贝类中消除病毒方面的价值，在这项研究中，我们通过实验污染研究了养殖贻贝中轮状病毒的积累和净化动力学。净化过程监测了 9 天，并在净化的第 1、2、3、5、7、9 天通过实时定量聚合酶链反应、细胞培养和电子显微镜评估病毒的残留存在和感染力。还分析了臭氧和微藻喂养等变量，作为可能的净化增强剂。结果表明，病毒去除动力学呈两相，在净化的最初 24 小时内有一个高降幅，5 天即可完全去除轮状病毒。
