Unit of Virology, Department of Animal Health, Zooprofilactic and Experimental Institute of Southern Italy, Via Salute n. 2, Portici (Naples), Italy.
CAISIAL-Aquaculture division, University of Naples Federico II, Via Università n.133, Portici (Naples), Italy.
Food Environ Virol. 2021 Sep;13(3):329-336. doi: 10.1007/s12560-021-09470-4. Epub 2021 Mar 17.
Kinetics of hepatitis A virus (HAV) accumulation and depuration from mussels (Mytilus galloprovincialis) was studied in an experimental depuration system. Different parameters likely to influence the rate of virus accumulation and elimination were evaluated. Analyses were carried out by both real-time RT-qPCR and digital PCR. Results demonstrated that the animals start to concentrate the virus already after one hour and reach the maximum level of contamination in 6 h of experiment. With respect to depuration, HAV showed a rapid reduction of the concentration (89%) during the first 24-48 h of experiment and a very slow virus decrement in the following days with a 1% residual RNA at the ninth day of depuration. When process parameters likely to increase the depuration rate (presence of ozone, microalgal feeding, presence of lactic bacteria, pre-treatment with digestive enzymes) were tested, no significant differences in the kinetics were observed. Only treatment with pancreatin seemed to positively affect depuration in the first two days of the experiment.
在一个实验净化系统中研究了甲型肝炎病毒 (HAV) 在贻贝 (Mytilus galloprovincialis) 中的积累和净化动力学。评估了可能影响病毒积累和消除速度的不同参数。通过实时 RT-qPCR 和数字 PCR 进行了分析。结果表明，动物在实验 1 小时后开始浓缩病毒，在 6 小时后达到污染的最高水平。就净化而言，HAV 在实验的头 24-48 小时内迅速减少浓度（89%），随后几天病毒减少速度非常缓慢，在净化的第 9 天仍有 1%的 RNA 残留。当测试可能提高净化率的过程参数（臭氧存在、微藻喂养、乳酸菌存在、消化酶预处理）时，动力学没有明显差异。只有用胰蛋白酶处理似乎在实验的头两天对净化有积极影响。
