抗精子抗体酶联免疫吸附测定（ELISA）试剂盒的临床评估

Clinical evaluation of the enzyme-linked immunosorbent assay (ELISA) kit for antisperm antibodies.

作者信息

Saji F, Ohashi K, Kato M, Negoro T, Tanizawa O

机构信息

Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.

出版信息

Fertil Steril. 1988 Oct;50(4):644-7. doi: 10.1016/s0015-0282(16)60199-1.

DOI:10.1016/s0015-0282(16)60199-1

PMID:3169285

Abstract

A commercially available enzyme-linked immunosorbent assay (ELISA) kit for the detection of antisperm antibodies in serum was compared with standard sperm immobilization test (SIT) with the use of sera from 83 infertile women and 29 control individuals. For the ELISA, 24% of the infertile patients and 10% of the controls showed positive results, whereas 15% of the patients and none of the control were positive in the SIT. Parallel tests carried out on the same sera indicated that these methods detect a different, though often overlapping, spectrum of antibody activity. The presence and number of motile sperm in cervical mucus during postcoital tests were found to be related to the results of the SIT. On the other hand, the ELISA did not appear to be related to the quality of postcoital tests. These data indicate that care must be employed to interpret the results of this ELISA kit for the detection of antisperm antibodies.

摘要

采用83例不孕女性和29例对照个体的血清，将一种用于检测血清中抗精子抗体的市售酶联免疫吸附测定（ELISA）试剂盒与标准精子制动试验（SIT）进行比较。对于ELISA，24%的不孕患者和10%的对照个体结果呈阳性，而对于SIT，15%的患者呈阳性，对照个体均为阴性。对相同血清进行的平行试验表明，这些方法检测的抗体活性谱不同，尽管常有重叠。发现在性交后试验期间宫颈黏液中活动精子的存在和数量与SIT的结果相关。另一方面，ELISA似乎与性交后试验的质量无关。这些数据表明，在解释此ELISA试剂盒检测抗精子抗体的结果时必须谨慎。