Suginami H, Yano K
Department of Obstetrics and Gynecology, Ehime University School of Medicine, Japan.
Fertil Steril. 1988 Oct;50(4):648-53. doi: 10.1016/s0015-0282(16)60200-5.
The present study was conducted to assess the mechanism of in vitro interference with fimbrial ovum capture by the ovum capture inhibitor (OCI) which we have recently demonstrated in endometriosis peritoneal fluid (PF). A golden hamster oviduct exposed to either endometriosis or nonendometriosis PF for 20 minutes at 37 degrees C was examined by scanning electron microscopy. Exposure of the oviduct to endometriosis PF reduced fimbrial activity of ovum capture and developed an OCI-related membrane on the fimbria, by which fimbrial cilia were completely concealed. This was not the case for nonendometriosis PF. Subsequently, an oviduct having been exposed to endometriosis PF was retrogradely flushed, by which the OCI-related membrane was ballooned and removed. The flushed oviduct resumed its activity of ovum capture. The OCI-related membrane appeared a cause of OCI interference with fimbrial ovum capture by preventing the contact between the fimbrial cilia and the cumulus oophorus.
本研究旨在评估卵子捕获抑制剂（OCI）体外干扰输卵管伞端卵子捕获的机制，我们最近在子宫内膜异位症腹腔液（PF）中证实了该抑制剂的存在。通过扫描电子显微镜检查，将金黄仓鼠输卵管在37℃下暴露于子宫内膜异位症或非子宫内膜异位症PF中20分钟。将输卵管暴露于子宫内膜异位症PF会降低输卵管伞端卵子捕获的活性，并在伞端形成与OCI相关的膜，通过该膜输卵管纤毛被完全遮盖。非子宫内膜异位症PF则不会出现这种情况。随后，对暴露于子宫内膜异位症PF的输卵管进行逆行冲洗，由此与OCI相关的膜膨胀并被清除。冲洗后的输卵管恢复了卵子捕获活性。与OCI相关的膜似乎是OCI通过阻止输卵管纤毛与卵丘之间的接触而干扰输卵管伞端卵子捕获的原因。
